机构：[1]BEIJING NEUROSURG INST,BEIJING 100050,PEOPLES R CHINA;研究所北京市神经外科研究所首都医科大学附属天坛医院[2]CHINESE ACAD PREVENT MED,INST OCCUPAT MED,29 NAN WEI RD,BEIJING 100050,PEOPLES R CHINA
In the present study, the changes of glial fibrillary acidic protein (GFAP) and microfilaments (MF) in primary cultured astrocytes were observed by using the fluorescence microscopy. Our results indicated that 1) hypothermia caused break-down of the cross-bridges among GFAP bundles but not depolymerization of GFAP itself; Whereas 2) the cross-linkings among MF bundles were broken down and MF itself depolymerized progressively; 3) GFAP in primary cultured astrocytes had a higher tolerance to hypothermia than MF; 4) The morphological changes of GFAP and MF were not only dependent on the temperature and exposure time, but also on the extent of cellular morphological differentiation. In brief, the above-mentioned changes of cytoskeletons may affect various biological processes related to the network of GFAP and MF.
第一作者机构：[1]BEIJING NEUROSURG INST,BEIJING 100050,PEOPLES R CHINA;[2]CHINESE ACAD PREVENT MED,INST OCCUPAT MED,29 NAN WEI RD,BEIJING 100050,PEOPLES R CHINA
通讯作者：
通讯机构：[1]BEIJING NEUROSURG INST,BEIJING 100050,PEOPLES R CHINA;[2]CHINESE ACAD PREVENT MED,INST OCCUPAT MED,29 NAN WEI RD,BEIJING 100050,PEOPLES R CHINA
推荐引用方式(GB/T 7714)：
JI HL,LI TL,WANG TY,et al.MORPHOLOGICAL CHANGE OF CYTOSKELETONS IN PRIMARY CULTURED ASTROCYTES AFTER COOLING[J].CRYOLETTERS.1995,16(4):209-214.
APA：
JI, HL,LI, TL,WANG, TY&LI, YR.(1995).MORPHOLOGICAL CHANGE OF CYTOSKELETONS IN PRIMARY CULTURED ASTROCYTES AFTER COOLING.CRYOLETTERS,16,(4)
MLA：
JI, HL,et al."MORPHOLOGICAL CHANGE OF CYTOSKELETONS IN PRIMARY CULTURED ASTROCYTES AFTER COOLING".CRYOLETTERS 16..4(1995):209-214
