机构：[1]College of Mechanical Engineering, Dongguan University of Technology, 1 Daxue Road, Dongguan, Guangdong 523808, China[2]Department of Pharmaceutical Sciences, State University of New York at Buffalo, 285 Kapoor Hall, Buffalo, New York 14260, United States[3]Center for Quantitative Sciences, Department of Biostatistics, Vanderbilt University School of Medicine, 2220 Pierce Avenue, Nashville, Tennessee 37232, United States[4]Model Animal Research Center, Nanjing University, Nanjing 210093, China[5]Department of Neurosurgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China临床科室神经外科首都医科大学附属安贞医院[6]Department of Internal Medicine, University of New Mexico, Comprehensive Cancer Center, Albuquerque, New Mexico 87131, United States[7]Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada[8]Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada
Failure to properly repair damaged due to myocardial infarction is a major cause of heart failure. In contrast with adult mammals, zebrafish hearts show remarkable regenerative capabilities after substantial damage. To characterize protein dynamics during heart regeneration, we employed an HPLC-ESI-MS/MS (mass spectrometry) approach. Myocardium tissues were taken from sham operated fish and ventricle-resected sample at three different time points (2, 7, and 14 days); dynamics of protein expression were analyzed by an ion-current-based quantitative platform. More than 2000 protein groups were quantified in all 16 experiments. Two hundred and nine heart-regeneration-related protein groups were quantified and clustered into six time-course patterns. Functional analysis indicated that multiple molecular function and metabolic pathways were involved in heart regeneration. Interestingly, Ingenuity Pathway Analysis revealed that P53 signaling was inhibited during the heart regeneration, which was further verified by real-time quantitative polymerase chain reaction (QPCR). In summary, we applied systematic proteomics analysis on regenerating zebrafish heart, uncovered the dynamics of regenerative genes expression and regulatory pathways, and provided invaluable insight into design regenerative-based strategies in human hearts.
基金：
Natural Sciences and Engineering Research Council of CanadaNatural Sciences and Engineering Research Council of Canada [RGPIN 341545]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [NSFC 31671505, NSFC 31471354]; Dongguan University of Technology innovation team startup [KCYXPT2016003]; Dongguan University of Technology faculty start-up [G200906-05]
第一作者机构：[1]College of Mechanical Engineering, Dongguan University of Technology, 1 Daxue Road, Dongguan, Guangdong 523808, China
共同第一作者：
通讯作者：
通讯机构：[1]College of Mechanical Engineering, Dongguan University of Technology, 1 Daxue Road, Dongguan, Guangdong 523808, China[4]Model Animal Research Center, Nanjing University, Nanjing 210093, China
推荐引用方式(GB/T 7714)：
Ma Danjun,Tu Chengan,Sheng Quanhu,et al.Dynamics of Zebrafish Heart Regeneration Using an HPLC-ESI-MS/MS Approach[J].JOURNAL OF PROTEOME RESEARCH.2018,17(3):1300-1308.doi:10.1021/acs.jproteome.7b00915.
APA：
Ma, Danjun,Tu, Chengan,Sheng, Quanhu,Yang, Yuxi,Kan, Zhisheng...&Lou, Xin.(2018).Dynamics of Zebrafish Heart Regeneration Using an HPLC-ESI-MS/MS Approach.JOURNAL OF PROTEOME RESEARCH,17,(3)
MLA：
Ma, Danjun,et al."Dynamics of Zebrafish Heart Regeneration Using an HPLC-ESI-MS/MS Approach".JOURNAL OF PROTEOME RESEARCH 17..3(2018):1300-1308
