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Microwave Tissue Dehydration A Fast and Reliable Tissue-Processing Method in Pathology

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收录情况: ◇ SCIE

机构: [1]Capital Univ Med Sci, Beijing Anzhen Hosp, Dept Pathol, 2 Anzhen Rd, Beijing 100029, Peoples R China
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关键词: conventional tissue processing histology histotechnology immunohistochemistry microwave tissue dehydration rapid tissue processing staining tissue stains tissue

摘要:
OBJECTIVE: To compare the histologic quality of tissues processed with rapid microwave tissue dehydration and traditional tissue dehydration. STUDY DESIGN: A total of 200 paired tissues from 15 organs were taken and divided into 2 groups. One group was processed with rapid microwave tissue dehydration, and the other one was processed with traditional tissue dehydration. Hematoxylin and eosin (H&E), immunohistochemical (IHC), and in situ hybridization (ISH) staining were conducted, and the staining qualities were compared carefully. Evaluation of blocks, H&E stain, IHC stain, and ISH were carried out. RESULTS: The quality of blocks and H&E stain showed no distinction. The IHC signal-positive location, percentage of positive cells, grades of intensity, and the staining background showed a very high concordance between the 2 groups. The ISH results show no distinction from each other. Moreover, microwave tissue dehydration is time-saving. CONCLUSION: As a rapid and reliable tissue processing method, microwave tissue dehydration may potentially be useful when rapid pathological diagnosis is needed.

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中科院(CAS)分区:
出版当年[2017]版:
大类 | 4 区 医学
小类 | 4 区 细胞生物学
最新[2023]版:
大类 | 4 区 医学
小类 | 4 区 细胞生物学
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出版当年[2016]版:
Q4 CELL BIOLOGY
最新[2023]版:

影响因子: 最新[2023版] 最新五年平均 出版当年[2016版] 出版当年五年平均 出版前一年[2015版] 出版后一年[2017版]

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第一作者机构: [1]Capital Univ Med Sci, Beijing Anzhen Hosp, Dept Pathol, 2 Anzhen Rd, Beijing 100029, Peoples R China
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