OBJECTIVE: To compare the histologic quality of tissues processed with rapid microwave tissue dehydration and traditional tissue dehydration. STUDY DESIGN: A total of 200 paired tissues from 15 organs were taken and divided into 2 groups. One group was processed with rapid microwave tissue dehydration, and the other one was processed with traditional tissue dehydration. Hematoxylin and eosin (H&E), immunohistochemical (IHC), and in situ hybridization (ISH) staining were conducted, and the staining qualities were compared carefully. Evaluation of blocks, H&E stain, IHC stain, and ISH were carried out. RESULTS: The quality of blocks and H&E stain showed no distinction. The IHC signal-positive location, percentage of positive cells, grades of intensity, and the staining background showed a very high concordance between the 2 groups. The ISH results show no distinction from each other. Moreover, microwave tissue dehydration is time-saving. CONCLUSION: As a rapid and reliable tissue processing method, microwave tissue dehydration may potentially be useful when rapid pathological diagnosis is needed.
第一作者机构:[1]Capital Univ Med Sci, Beijing Anzhen Hosp, Dept Pathol, 2 Anzhen Rd, Beijing 100029, Peoples R China
推荐引用方式(GB/T 7714):
Li Yanwei,Mei Shaoshuai,Cui Yayan,et al.Microwave Tissue Dehydration A Fast and Reliable Tissue-Processing Method in Pathology[J].ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY.2018,40(2):63-68.
APA:
Li, Yanwei,Mei, Shaoshuai,Cui, Yayan,Teng, Fei&Wang, Wei.(2018).Microwave Tissue Dehydration A Fast and Reliable Tissue-Processing Method in Pathology.ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY,40,(2)
MLA:
Li, Yanwei,et al."Microwave Tissue Dehydration A Fast and Reliable Tissue-Processing Method in Pathology".ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY 40..2(2018):63-68