Increased inflammation, endoplasmic reticulum stress and oxidative stress in endothelial and macrophage cells exacerbate atherosclerosis in ApoCIII transgenic mice
机构：[1]Beijing Anzhen Hospital of Capital Medical University and Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.首都医科大学附属安贞医院[2]Department of Endocrinology, Beijing Puren Hospital, Beijing 100062, China.[3]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.首都医科大学附属同仁医院
Background: Overexpression of apolipoprotein CIII (ApoCIII) leads to hypertriglyceridemia (HTG) which promotes atherosclerosis development. However, it remains unclear whether ApoCIII affects the atherosclerosis alone by promoting the inflammation and endoplasmic reticulum (ER) stress, or in combination with HTG. Methods: Transgenic (ApoCIIItg) mouse models were used to investigate the atherogenic role of ApoCIII. Since endothelial cells and macrophages play crucial roles in atherosclerosis, we examined whether triglyceride-rich lipoproteins (TRLs), the major lipoproteins, in plasma of ApoCIIItg mice affect inflammation and ER stress levels in these cells. To further investigate the role of ApoCIII and triglyceride, we incubated HUVECs cells and peritoneal macrophages with TRLs with or without ApoCIII. Results: Increased inflammation and ER stress were found in the aorta of ApoCIIItg mice. TRLs increased ER stress and oxidative stress in HUVECs and macrophages in a dose dependent. Moreover, TRLs together with ApoCIII could induce a higher inflammation level than TRLs alone in these cells. Conclusions: Both TRLs and ApoCIII contribute to the progression of atherosclerosis, and the modulation of TRLs and ApoCIII may represent a novel therapeutic approach against HTG induced atherosclerosis.
基金：
National Natural Science Foundation for Young Scientists [81300228]; Excellent Scholars of Beijing Dongcheng District [2014074181449G008]
第一作者机构：[1]Beijing Anzhen Hospital of Capital Medical University and Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
通讯作者：
通讯机构：[3]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
推荐引用方式(GB/T 7714)：
Han Yingchun,Ma Yahong,Wen Jiangping,et al.Increased inflammation, endoplasmic reticulum stress and oxidative stress in endothelial and macrophage cells exacerbate atherosclerosis in ApoCIII transgenic mice[J].LIPIDS IN HEALTH AND DISEASE.2018,17(1):-.doi:10.1186/s12944-018-0867-5.
APA：
Han Yingchun,Ma Yahong,Wen Jiangping,He Xiaokui&Zhang Xiaohong.(2018).Increased inflammation, endoplasmic reticulum stress and oxidative stress in endothelial and macrophage cells exacerbate atherosclerosis in ApoCIII transgenic mice.LIPIDS IN HEALTH AND DISEASE,17,(1)
MLA：
Han Yingchun,et al."Increased inflammation, endoplasmic reticulum stress and oxidative stress in endothelial and macrophage cells exacerbate atherosclerosis in ApoCIII transgenic mice".LIPIDS IN HEALTH AND DISEASE 17..1(2018):-
