Dendritic cells (DC) are the most potent antigen-presenting cells for initiating immune responses. DC maturation can be induced by exposing of immature DC to pathogen products or pro-inflammatory factor, which dramatically enhances the ability of DC to activate Ag-specific T cells. In this study, a recombinant calreticulin fragment 39-272 (rCRT/39-272) covering the lectin-like N domain and partial P domain of murine CRT has been expressed and purified in Escherichia coli. Functional analysis studies revealed that rCRT/39-272 has potent immunostimulatory activities in both activating human monocytes and B cells to secrete cytokines. rCRT/39-272 can drive the activation of bone marrow derived DC in TLR4/CD14 dependent way, as indicated by secretion of cytokines IL-12/IL-23 (p40) and IL-1 beta. Exposure of DC to rCRT/39-272 induces P-Akt, suggesting that rCRT/39-272 induces maturation of DC through PI3K/Akt signaling pathway. The results suggest that soluble rCRT/39-272 is a potent stimulatory agent to DC maturation in TLR4/CD14 and PI3K/Akt dependent pathway. It may play important roles in initiating cellular immunity in vivo and the T cell response in vitro. Thus it could be used for study of DC-based tumor vaccines.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30890142, 31070781]; Priority Academic Program Development of Jiangsu Province Higher Education Institutions; National Key Basic Research ProgramsNational Basic Research Program of China [2007CB512406, 2010CB529102]
第一作者机构：[1]Capital Med Univ, Beijing Anzhen Hosp, Beijing 100029, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Anzhen Hosp, Beijing 100029, Peoples R China;[2]Capital Med Univ, Beijing Anzhen Hosp, Chaoyang Dist Anzhen Rd 2, Beijing 100029, Peoples R China
推荐引用方式(GB/T 7714)：
Li Yue,Zeng Xiaoli,He Lijuan,et al.Dendritic cell activation and maturation induced by recombinant calreticulin fragment 39-272[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE.2015,8(5):7288-7296.
APA：
Li, Yue,Zeng, Xiaoli,He, Lijuan&Yuan, Hui.(2015).Dendritic cell activation and maturation induced by recombinant calreticulin fragment 39-272.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,8,(5)
MLA：
Li, Yue,et al."Dendritic cell activation and maturation induced by recombinant calreticulin fragment 39-272".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 8..5(2015):7288-7296
