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Identification of a cancer stem cell-like side population in the HeLa human cervical carcinoma cell line

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收录情况： ◇ SCIE

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机构： [1]Capital Med Univ, Dept Obstet & Gynecol, Beijing Anzhen Hosp, Beijing 100029, Peoples R China; [2]Chinese Acad Med Sci, Dept Obstet & Gynecol, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China; [3]Chinese Acad Med Sci, Dept Pathol, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China; [4]Chinese Acad Med Sci, Dept Obstet & Gynecol, Peking Union Med Coll Hosp, 9 Dongdan Santiao, Beijing 100730, Peoples R China

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关键词： cervical carcinoma HeLa cell line side population cells cancer stem cells CD133

摘要：

The present study aimed to identify the stem cell characteristics of side population (SP) cells sorted from the widely-used HeLa human cervical carcinoma cell line. The SP cells were sorted from the HeLa cell line using fluorescence-activating cell sorting (FACS). Stem cell characteristics of the SP cells, including proliferation, self-renewal, differentiation and the ability to form xenografts, were investigated in vitro and in vivo. The SP cells demonstrated strong tumorigenesis following in vivo transplantation into five to six-week-old female Balb/c mice. The SP cells were observed to be more resistant to chemotherapy and radiotherapy compared with non-side population (NSP) cells. A higher expression of CD133 was observed in the SP cells compared with the NSP cells following FACS. The results demonstrated that the SP cells from the HeLa human cervical carcinoma cell line exhibit stem cell characteristics in vitro and also have a strong ability to form tumors in vivo. The cell surface marker CD133 may serve as a potential molecular marker for the identification of cervical cancer stem cells (CSCs).

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中科院(CAS)分区：

出版当年[2012]版：

大类 | 4 区医学

小类 | 4 区肿瘤学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区肿瘤学

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出版当年[2011]版：

Q4 ONCOLOGY

最新[2023]版：

Q3 ONCOLOGY

影响因子： 2.5 最新[2023版] 2.4 最新五年平均 0.108 出版当年[2011版] 0.108 出版当年五年平均 0 出版前一年[2010版] 0.237 出版后一年[2012版]

第一作者：

第一作者机构： [1]Capital Med Univ, Dept Obstet & Gynecol, Beijing Anzhen Hosp, Beijing 100029, Peoples R China;

通讯作者：

通讯机构： [2]Chinese Acad Med Sci, Dept Obstet & Gynecol, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China; [4]Chinese Acad Med Sci, Dept Obstet & Gynecol, Peking Union Med Coll Hosp, 9 Dongdan Santiao, Beijing 100730, Peoples R China

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