详情页 - 首都医科大学宣武医院知识库

当前位置：首页 > 详情页

LINC00667 promotes Wilms' tumor metastasis and stemness by sponging miR-200b/c/429 family to regulate IKK-β.

文献详情

资源类型：

作者：

机构： [1]Department of Pediatric Urology, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, No. 56 Nanlishilu Street, Xicheng District, 100045, Beijing, China

出处：

DOI：

ISSN：

关键词： IKK-β LINC00667 miR-200b/c/429 NF-κB pathway Wilms' tumor

摘要：

Wilms' tumor, also known as nephroblastoma, is a kind of pediatric renal cancer. Previous studies have indicated that microRNAs (miRNAs) regulate various cancers progression. However, whether miR-200 family regulated Wilms' tumor progression remains to be elucidated. In our study, miR-200b/c/429 expression was down-regulated in Wilms' tumor tissue samples from 25 patients. And data from three independent analyses of RT-qPCR revealed that the expression of miR-200b/c/429 was down-regulated in Wilms' tumor cell lines. Functionally, CCK-8 assay revealed that cell viability was reduced by overexpressing miR-200b/c/429. Transwell assay manifested that cell migration and invasion was hindered by miR-200b/c/429 overexpression. Sphere-forming and Western blot assays demonstrated that miR-200b/c/429 overexpression suppressed sphere formation ability. Mechanically, NF-κB pathway was confirmed to be associated with Wilms' tumor progression, miR-200b/c/429 overexpression inactivated NF-κB pathway as miR-200b/c/429 was identified to target IKK-β, a NF-κB pathway related gene. Moreover, miR-200b/c/429 was sponged by LINC00667 in Wilms' tumor cells. LINC00667 competitively bound with miR-200b/c/429 to regulate IKK-β expression and then activate NF-κB pathway in Wilms' tumor. Subsequently, rescue assays illustrated silence of IKK-β could reverse the effect of miR-200b/c/429 inhibition on the progression of sh-LINC00667-transfected Wilms' tumor cells. In summary, LINC00667 promoted Wilms' tumor progression by sponging miR-200b/c/429 family to regulate IKK-β. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

语种：

PubmedID：

中科院(CAS)分区：

出版当年[2019]版：

大类 | 4 区生物

小类 | 4 区细胞生物学

最新[2023]版：

大类 | 3 区生物学

小类 | 4 区细胞生物学

第一作者：

第一作者机构： [1]Department of Pediatric Urology, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, No. 56 Nanlishilu Street, Xicheng District, 100045, Beijing, China

通讯作者：

通讯机构： [1]Department of Pediatric Urology, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, No. 56 Nanlishilu Street, Xicheng District, 100045, Beijing, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]核因子κB信号通路在肺癌中的作用 [2]Fingolimod Alleviates Inflammation after Cerebral Ischemia via HMGB1/TLR4/NF-κB Signaling Pathway [3]NMO-IgG Induce Interleukin-6 Release via Activation of the NF-κB Signaling Pathway in Astrocytes. [4]Polygalacic acid attenuates cognitive impairment by regulating inflammation through PPARγ/NF-κB signaling pathway [5]Analgecine regulates microglia polarization in ischemic stroke by inhibiting NF-κB through the TLR4 MyD88 pathway. [6]Tenuifolin Attenuates Amyloid-β42-Induced Neuroinflammation in Microglia Through the NF-κB Signaling Pathway. [7]核因子κB信号通路为靶点进行非小细胞肺癌治疗的研究进展 [8]IVIG suppresses the up-regulation of the NF-κB/MMP-9 pathway in a mouse model of Kawasaki disease [9]儿童囊性肾瘤和囊性部分分化型肾母细胞瘤的诊治分析 [10]Wilms' Tumor 1 (WT1): The Vaccine for Cancer.