机构：[1]Department of Ophthalmology,Xuanwu Hospital, Capital Medical University, Beijing, China外科系统眼科首都医科大学宣武医院[2]Department of Neurology and China-America Institute of Neuroscience,Xuanwu Hospital, Capital Medical University, Beijing, China神经科系统科技平台神经内科中美神经科学研究所首都医科大学宣武医院[3]Institute of Hypoxia Medicine,Xuanwu Hospital, Capital Medical University, Beijing, China首都医科大学宣武医院低氧医学研究室[4]Department of Biomedical Engineering, School of Biological Science and Medical Engineering,Beihang University, Beijing, China[5]Beijing Advanced Innovation Center for Big Data-based Precision Medicine,Beihang University, Beijing, China[6]Department of Neurosurgery,Wayne State University School of Medicine, Detroit.
BACKGROUND AND PURPOSE: The retina, as an externally located neural tissue, offers unique advantages in investigating the effect of therapeutic intervention on the brain. In this study, we put forth a clinically relevant model of retinal ischemia and reperfusion in nonhuman primates. METHODS: Acute retinal artery ischemia and reperfusion was induced by injecting an autologous clot into the ophthalmic artery of adult rhesus monkeys, and recanalization was achieved by focal thrombolysis with tPA (tissue-type plasminogen activator). Digital subtraction angiography and fluorescein angiography were used to evaluate blood flow in the retina and the choroid. Electroretinogram, optical coherence tomography, and hematoxylin and eosin staining were used to evaluate the structure and function of the retina after ischemia. RESULTS: Digital subtraction angiography and fluorescein angiography images confirmed occlusion of the ophthalmic and central retinal arteries, as well as recanalization after tPA thrombolysis. Electroretinogram indicated retinal functional damage following ischemia, and thrombolysis partially rescued its impairment. Optical coherence tomography and hematoxylin and eosin staining revealed ischemia-induced changes in the retina, and tPA partially mitigated these damages. CONCLUSIONS: This novel acute retinal artery ischemia and reperfusion model in rhesus monkeys may closely simulate retinal ischemia/reperfusion in clinical practice and provide an optimal platform for screening neuroprotective strategies.
基金：
This work was supported by the National Natural Science Foundation of China (81620108011, 81871022, and 81771260), Beijing Municipal Science and Technology Commission, Capital Clinical Characteristics Application Research
Special General Topic (Z171100001017029).
第一作者机构：[1]Department of Ophthalmology,Xuanwu Hospital, Capital Medical University, Beijing, China[4]Department of Biomedical Engineering, School of Biological Science and Medical Engineering,Beihang University, Beijing, China[5]Beijing Advanced Innovation Center for Big Data-based Precision Medicine,Beihang University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurology and China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing 100053, China[*2]Department of Ophthalmology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Yuan Gao,Di Wu,Dachuan Liu,et al.Novel Acute Retinal Artery Ischemia and Reperfusion Model in Nonhuman Primates[J].STROKE.2020,51(8):2568-2572.doi:10.1161/STROKEAHA.119.028809.
APA：
Yuan Gao,Di Wu,Dachuan Liu,Mitchell Huber,Jian Chen...&Xuxiang Zhang.(2020).Novel Acute Retinal Artery Ischemia and Reperfusion Model in Nonhuman Primates.STROKE,51,(8)
MLA：
Yuan Gao,et al."Novel Acute Retinal Artery Ischemia and Reperfusion Model in Nonhuman Primates".STROKE 51..8(2020):2568-2572
医学