机构：[1]Department of Vascular Ultrasonography, Xuanwu Hospital, Capital Medical University, China医技科室血管超声科首都医科大学宣武医院[2]Beijing Diagnostic Center of Vascular Ultrasound, China[3]Center of Vascular Ultrasonography, Beijing Institute of Brain Disorders, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing, China. 45 Changchun Road, Xicheng District, Beijing, 100053, China[4]Beijing Sinogene Biotechnology Co. Ltd, Beijing, 102200, China[5]Department of Physiology and Pathophysiology, School of Basic Medical Science, Capital Medical University, Beijing, 100069, China
Background and aims: This study evaluated the atherosclerosis phenotype by vascular duplex ultraso-nography (VDU) in ApoE-deficient dogs. Methods: A total of 108 beagle dogs were examined by VDU, which included 32 wild-type, 68 hetero-zygous (ApoE-/+) mutant and 8 homozygous (ApoE-/-) mutant dogs. According to age, wild-type and ApoE-/+ dogs were divided into two subgroups: young (6-15 months) and adult dogs (18-29 months). All homozygous dogs were young dogs. Dogs were feed with normal diet. The plasma lipid levels were tested. The diameter of the common carotid artery (CCA), internal carotid artery (ICA), external carotid artery (ECA), abdominal aorta and common iliac artery (CIA) and the intima-media thickness (IMT) of the CCA and abdominal aorta were measured by VDU. The artery sections of ApoE-/-and control dogs were analyzed by histological analysis. Results: The plasma triglycerides (2.5-3 fold), total cholesterol (4-5 fold) and LDL levels (35-40 fold) of ApoE-/-dogs were higher than those of the wild-type and ApoE-/+ dogs. Compared with the wild-type and young ApoE-/+ dogs, the IMT of CCA and aorta in ApoE-/-dogs were increased (p<0.05). The occurrence of atherosclerosis in ApoE-/-dogs was higher than that in ApoE-/+ dogs (50% vs. 10.3%, p=0.013) and the occurrence time was earlier. Histology confirmed that the aorta, carotid arteries and CIA had atherosclerotic lesions in ApoE-/-dogs. Conclusions: The ApoE-deficient dogs were a reliable animal model of atherosclerosis. VDU is an optimal in vivo noninvasive method for evaluating atherosclerosis phenotypes in large animal models. (c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
第一作者机构：[1]Department of Vascular Ultrasonography, Xuanwu Hospital, Capital Medical University, China[2]Beijing Diagnostic Center of Vascular Ultrasound, China[3]Center of Vascular Ultrasonography, Beijing Institute of Brain Disorders, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing, China. 45 Changchun Road, Xicheng District, Beijing, 100053, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Vascular Ultrasonography, Xuanwu Hospital, Capital Medical University, China[2]Beijing Diagnostic Center of Vascular Ultrasound, China[3]Center of Vascular Ultrasonography, Beijing Institute of Brain Disorders, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing, China. 45 Changchun Road, Xicheng District, Beijing, 100053, China[*1]Xuanwu Hospital, Address: No. 45 Changchun Road, Beijing, 100053, China.
推荐引用方式(GB/T 7714)：
Jia Lingyun,Li Yuan,Hua Yang,et al.Identification of the atherosclerosis phenotype in vivo by vascular duplex ultrasonography in ApoE-deficient dogs[J].ATHEROSCLEROSIS PLUS.2022,47:8-15.doi:10.1016/j.athplu.2021.12.001.
APA：
Jia, Lingyun,Li, Yuan,Hua, Yang,Liu, Yumei,Zhang, Nan...&Jiao, Shiyu.(2022).Identification of the atherosclerosis phenotype in vivo by vascular duplex ultrasonography in ApoE-deficient dogs.ATHEROSCLEROSIS PLUS,47,
MLA：
Jia, Lingyun,et al."Identification of the atherosclerosis phenotype in vivo by vascular duplex ultrasonography in ApoE-deficient dogs".ATHEROSCLEROSIS PLUS 47.(2022):8-15
