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The landscape of PBMCs in AQP4-IgG seropositive NMOSD and MOGAD, assessed by high dimensional mass cytometry

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机构： [1]Department of Neuroinfection and Neuroimmunology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. [2]China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. [3]Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical University, Beijing, China. [4]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China. [5]Collaborative Innovation Center for Brain Disorders, Beijing Institute of Brain Disorders, Capital Medical University, Beijing, China.

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关键词： aquaporin-4-IgG seropositive NMOSD mass cytometry MOGAD PBMCs

摘要：

Data on peripheral blood mononuclear cells (PBMCs) characteristics of aquaporin-4 (AQP4)-IgG seropositive neuromyelitis optica spectrum disorder (NMOSD) and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) are lacking. In this study, we describe the whole PBMCs landscape of the above diseases using cytometry by time-of-flight mass spectrometry (CyTOF).The immune cell populations were phenotyped and clustered using CyTOF isolated from 27 AQP4-IgG seropositive NMOSD, 11 MOGAD patients, and 15 healthy individuals. RNA sequencing was employed to identify critical genes. Fluorescence cytometry and qPCR analysis were applied to further validate the algorithm-based results that were obtained.We identified an increased population of CD11b+ mononuclear phagocytes (MNPs) in patients with high expression of CCR2, whose abundance may correlate with brain inflammatory infiltration. Using fluorescence cytometry, we confirmed the CCR2+ monocyte subsets in a second cohort of patients. Moreover, there was a wavering of B, CD4+ T, and NKT cells between AQP4-IgG seropositive NMOSD and MOGAD.Our findings describe the whole landscape of PBMCs in two similar demyelinated diseases and suggest that, besides MNPs, T, NK and B, cells were all involved in the pathogenesis. The identified cell population may be used as a predictor for monitoring disease development or treatment responses.© 2024 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd.

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出版当年[2023]版：

大类 | 1 区医学

小类 | 2 区神经科学 2 区药学

最新[2025]版：

大类 | 2 区医学

小类 | 2 区药学 3 区神经科学

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出版当年[2022]版：

Q1 NEUROSCIENCES Q1 PHARMACOLOGY & PHARMACY

最新[2024]版：

Q1 NEUROSCIENCES Q1 PHARMACOLOGY & PHARMACY

影响因子： 5 最新[2024版] 5.8 最新五年平均 5.5 出版当年[2022版] 6.2 出版当年五年平均 7.035 出版前一年[2021版] 4.8 出版后一年[2023版]

第一作者：

第一作者机构： [1]Department of Neuroinfection and Neuroimmunology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. [2]China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

通讯作者：

通讯机构： [1]Department of Neuroinfection and Neuroimmunology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. [2]China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. [4]Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China. [5]Collaborative Innovation Center for Brain Disorders, Beijing Institute of Brain Disorders, Capital Medical University, Beijing, China.

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The landscape of PBMCs in AQP4-IgG seropositive NMOSD and MOGAD, assessed by high dimensional mass cytometry

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