机构：[1]Department of Respiration, The Second Affiliated Hospital of Soochow University, Suzhou, P.R. China,[2]Invitrocue Biomedical Service Suzhou, Suzhou, P.R. China,[3]Department of Thoracic & Cardiac Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, P.R. China,[4]Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou, P.R. China,[5]Department of Medical Oncology, Shanghai Pulmonary Hospital, Thoracic Cancer Institute, Tongji University School of Medicine, Shanghai, P. R. China
The prognosis of advanced non-small cell lung cancer (NSCLC) patients is poor. One of the reasons for this hampered progress has been a lack of in vitro models that would faithfully recapitulate the heterogeneity of tumors and response to treatment. In this study, surgically resected tumors were obtained from patients with stage I/II NSCLC during curative-intent surgery. Using a 3D patient-derived tumor spheroids culture system, our results demonstrate successful long-term expansion of primary NSCLC cells in vitro (> 120 days). Patient derived tumor spheroid (PDS) cultures could be established with a success rate of 100% (3 out of 3 samples). Consistent with their growth in culture and their cancer type, many cells within the tumor spheroids were stained positive for Ki67 and thyroid transcription factor-1. The result of this study supports the establishment of an expandable 3D in vitro NSCLC model for drug screening, and enables the potential long term studies such as the establishment of drug resistant models.
基金：
This work was supported by the Second
Affiliated Hospital of Soochow University
Preponderant Clinic Discipline Group Project
Funding (XKQ2015OO5) and the Suzhou Municipal
Key Medical Disciplines (Respiratory Medicine)
grant (SZXK201506).
第一作者机构：[1]Department of Respiration, The Second Affiliated Hospital of Soochow University, Suzhou, P.R. China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Respiration, The Second Affiliated Hospital of Soochow University, Suzhou, P.R. China,[5]Department of Medical Oncology, Shanghai Pulmonary Hospital, Thoracic Cancer Institute, Tongji University School of Medicine, Shanghai, P. R. China
推荐引用方式(GB/T 7714)：
Zengli Zhang,Huiqian Wang,Qifeng Ding,et al.Establishment of patient-derived tumor spheroids for non-small cell lung cancer[J].PLOS ONE.2018,13(3):e0194016.doi:10.1371/journal.pone.0194016.
APA：
Zengli Zhang,Huiqian Wang,Qifeng Ding,Yufei Xing,Zhonghua Xu...&Minhua Shi.(2018).Establishment of patient-derived tumor spheroids for non-small cell lung cancer.PLOS ONE,13,(3)
MLA：
Zengli Zhang,et al."Establishment of patient-derived tumor spheroids for non-small cell lung cancer".PLOS ONE 13..3(2018):e0194016
