机构：[a]College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China[b]The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[c]Xuzhou Hospital of Traditional Chinese Medicine, Xuzhou 320300, China
The decoction of Pteris multifida had been applied to attenuate symptoms of benign prostatic hyperplasia in Chinese folk medicine. In this study, the total flavonoid extract of Pteris multifida was processed at first. High performance liquor chromatography and tandem mass spectrometer assay revealed 10 flavonoids as key constituents of this extract. After 60-day administration, the total flavonoid extract (180 mg/kg, i.g.) decreased the prostate index in mice of benign prostatic hyperplasia apparently. Immunohistochemical assay revealed inhibition of vascular endothelial growth factor expression, together with activation of transforming growth factor-beta 1 expression in the prostatic samples after administration of the extract. A 90-day subchronic toxicity test was further undertaken in male Sprague-Dawley rats, and the no-observed-adverse-effect level for the extract was 200 mg/kg body weight/day. These results revealed that the total flavonoid extract of Pteris multifida exhibited positive effect with safety, which might be applied in treatment of benign prostatic hyperplasia. (C) 2016 Elsevier Ltd. All rights reserved.
基金：
This work was supported by Jiangsu province science and technology
support program-social development projects (BE2011671,
BE2012649), and National Natural Science Foundation of China
(81274190).
第一作者机构：[a]College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China
通讯作者：
通讯机构：[a]College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China[b]The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
推荐引用方式(GB/T 7714)：
Guang-Cheng Dai,Bin Hu,Wen-Fang Zhang,et al.Chemical characterization, anti-benign prostatic hyperplasia effect and subchronic toxicity study of total flavonoid extract of Pteris multifida[J].FOOD AND CHEMICAL TOXICOLOGY.2017,108(Pt B):524-531.doi:10.1016/j.fct.2016.11.010.
APA：
Guang-Cheng Dai,Bin Hu,Wen-Fang Zhang,Fang Peng,Rong Wang...&Yu-Xi Shan.(2017).Chemical characterization, anti-benign prostatic hyperplasia effect and subchronic toxicity study of total flavonoid extract of Pteris multifida.FOOD AND CHEMICAL TOXICOLOGY,108,(Pt B)
MLA：
Guang-Cheng Dai,et al."Chemical characterization, anti-benign prostatic hyperplasia effect and subchronic toxicity study of total flavonoid extract of Pteris multifida".FOOD AND CHEMICAL TOXICOLOGY 108..Pt B(2017):524-531
