Aim: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella. Materials & methods: Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and Delta rpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and Delta rpoE strains was examined after 120 min of hyperosmotic stress. Results: SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the Delta rpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and Delta rpoE strains. Conclusion: RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.
基金：
This study was supported by the National Natural Science Foundation of China (no. 81572032, 31300122) and the Science and Technology Program of Suzhou (SYSD2014094, SYS201551).
第一作者机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China[2]School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China
推荐引用方式(GB/T 7714)：
Haifang Zhang,Yanwei Jia,Xiaofang Xie,et al.RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi[J].FUTURE MICROBIOLOGY.2016,11(8):1011-24.doi:10.2217/fmb.16.19.
APA：
Haifang Zhang,Yanwei Jia,Xiaofang Xie,Min Wang,Yi Zheng...&Hong Du.(2016).RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi.FUTURE MICROBIOLOGY,11,(8)
MLA：
Haifang Zhang,et al."RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi".FUTURE MICROBIOLOGY 11..8(2016):1011-24
