机构：[1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.首都医科大学宣武医院低氧医学研究室[2]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing, 100053, China.[3]Center of Stroke, Beijing Institute for Brain Disorder, Beijing 100069, China.[4]Department of Psychiatry, Center for Neuroproteomics and Biomarkers Research, University of Florida, Gainesville, Florida, USA.[5]Department of Biochemistry and MolecularGenetics, Faculty of Medicine, American University of Beirut Medical Center, Beirut, Lebanon.[6]Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA.[7]Faculty of Medicine, American University of Beirut Medical Center, Beirut, Lebanon.[8]Biology Department, Faculty of Sciences, Lebanese University, Beirut, Lebanon.[9]Banyan Labs, Banyan Biomarkers Inc., Alachua, FL, USA.[10]Department of Neurosurgery, Wayne State University, School of Medicine, Detroit, 48201, MI, USA.[11]Department of Biomedical, Dental and Morphological and Functional Imaging Sciences, University of Messina, Messina, Italy.
A rapid and reliable diagnostic test to distinguish ischemic from hemorrhagic stroke in patients presenting with stroke-like symptoms is essential to optimize management and triage for thrombolytic therapy. The present study measured serum concentrations of ubiquitin C-terminal hydrolase (UCH-L1) and glial fibrillary astrocytic protein (GFAP) in acute stroke patients and healthy controls and investigated their relation to stroke severity and patient characteristics. We also assessed the diagnostic performance of these markers for the differentiation of intracerebral hemorrhage (ICH) from ischemic stroke (IS). Both UCH-L1 and GFAP concentrations were significantly greater in ICH patients than in controls (p < 0.0001). However, exclusively GFAP differed in ICH compared with IS (p < 0.0001). GFAP yielded an AUC of 0.86 for differentiating between ICH and IS within 4.5hrs of symptom onset with a sensitivity of 61% and a specificity of 96% using a cut-off of 0.34ng/ml. Higher GFAP levels were associated with stroke severity and history of prior stroke. Our results demonstrate that blood UCH-L1 and GFAP are increased early after stroke and distinct biomarker-specific release profiles are associated with stroke characteristics and type. We also confirmed the potential of GFAP as a tool for early rule-in of ICH, while UCH-L1 was not clinically useful.
基金：
Natural Science Foundation of China [81573867]; Scientific Special Funding of Capital Health Development [2011-1001-03]; China National Funds for Distinguished Youth Scientist [81325007]; Distinguished Professor of cheung kong scholars programme [T2014251]; 12th Five Year Plan of National Science and Technology [2013BAI07B00]
第一作者机构：[1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.[2]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing, 100053, China.[3]Center of Stroke, Beijing Institute for Brain Disorder, Beijing 100069, China.
通讯作者：
通讯机构：[1]Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.[2]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing, 100053, China.[3]Center of Stroke, Beijing Institute for Brain Disorder, Beijing 100069, China.[11]Department of Biomedical, Dental and Morphological and Functional Imaging Sciences, University of Messina, Messina, Italy.
推荐引用方式(GB/T 7714)：
Changhong Ren,Firas Kobeissy,Ali Alawieh,et al.Assessment of Serum UCH-L1 and GFAP in Acute Stroke Patients[J].SCIENTIFIC REPORTS.2016,6(6):24588.doi:10.1038/srep24588.
APA：
Changhong Ren,Firas Kobeissy,Ali Alawieh,Na Li,Ning Li...&Stefania Mondello.(2016).Assessment of Serum UCH-L1 and GFAP in Acute Stroke Patients.SCIENTIFIC REPORTS,6,(6)
MLA：
Changhong Ren,et al."Assessment of Serum UCH-L1 and GFAP in Acute Stroke Patients".SCIENTIFIC REPORTS 6..6(2016):24588
