机构：[1]Department of Gastroenterology,Second Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang PR China,[2]Department of General Surgery,Xuan Wu Hospital, Capital Medical University,Beijing, PR China,普通外科首都医科大学宣武医院[3]Department of Gastroenterology,First Hospital of Harbin,Harbin, Heilongjiang PR China,[4]Department of Pathology, Second Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang PR China,[5]Department of Pathology, Harbin Medical University,Harbin, Heilongjiang PR China
NPRL2 is a tumor suppressor gene involved in the progression of human cancer. The present study investigated whether NPRL2 expression correlates with colorectal cancer (CRC) progression. Colorectal tissue and peripheral blood samples were obtained from 62 patients with CRC, 38 patients with colorectal adenomas and 51 normal controls. NPRL2 mRNA levels in tissue samples and blood were measured using quantitative real-time PCR. NPRL2 protein expression was determined by immunohistochemistry. NPRL2 protein expression in CRCs was significantly lower than in the adenomas or normal colorectal tissue. NPRL2 mRNA expression was significantly decreased in adenomas compared with normal controls (P < 0.0001) and it was further decreased in colorectal tumors compared with adenomas (P < 0.0001). NPRL2 mRNA levels expression correlated with tumor stage. In addition, NPRL2 mRNA levels in the blood correlated with the levels detected in tumors. Furthermore, receiver operating characteristic (ROC) analysis showed that NPRL2 expression in blood could distinguish colorectal adenomas and CRCs from normal controls. NPRL2 mRNA expression in CRC tumor tissues and peripheral blood correlated with colorectal tumor progression. Based on our findings, we can conclude that NPRL2 mRNA blood levels could be a potentially useful marker for the detection of early stage adenomas and CRCs.
第一作者机构：[1]Department of Gastroenterology,Second Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang PR China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Gastroenterology,Second Affiliated Hospital of Harbin Medical University,Harbin, Heilongjiang PR China,
推荐引用方式(GB/T 7714)：
Ai-yun Liu,Dian-gang Liu,Ya-ju Du,et al.Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer[J].CANCER BIOLOGY & THERAPY.2014,15(5):489-495.doi:10.4161/cbt.28016.
APA：
Ai-yun Liu,Dian-gang Liu,Ya-ju Du,Feng-hua Pei,Guang Yang...&Jing Chen.(2014).Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer.CANCER BIOLOGY & THERAPY,15,(5)
MLA：
Ai-yun Liu,et al."Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer".CANCER BIOLOGY & THERAPY 15..5(2014):489-495
