The Two Isomers of HDTIC Compounds from Astragali Radix Slow Down Telomere Shortening Rate via Attenuating Oxidative Stress and Increasing DNA Repair Ability in Human Fetal Lung Diploid Fibroblast Cells
机构：[1]Aging Research Center, Health and Science School of Peking University, Beijing, People’s Republic of China.[2]Medical Test Department, Xuanwu Hospital of Capital Medical University, Beijing, People’s Republic of China.首都医科大学宣武医院
4-Hydroxy-5-hydroxymethyl-[1,3]dioxolan-2,6'-spirane-5',6',7',8'-tetrahydro-indolizine-3'-carbaldehyde (HDTIC)-1 and HDTIC-2 are two isomers extracted from Astragalus membranaceus (Fisch) Bunge Var. mongholicus (Bge) Hsiao. Our previous study had demonstrated that they could extend the lifespan of human fetal lung diploid fibroblasts (2BS). To investigate the mechanisms of the HDTIC-induced delay of replicative senescence, in this study, we assessed the effects of these two compounds on telomere shortening rate and DNA repair ability in 2BS cells. The telomere shortening rates of the cells cultured with HDTIC-1 or HDTIC-2 were 31.5 and 41.1 bp with each division, respectively, which were much less than that of the control cells (71.1 bp/PD). We also found that 2BS cells pretreated with HDTIC-1 or HDTIC-2 had a significant reduction in DNA damage after exposure to 200 mu M H2O2 for 5 min. Moreover, the 100 mu M H2O2-induced DNA damage was significantly repaired after the damaged cells were continually cultured with HDTIC for 1 h. These results suggest that HDTIC compounds slow down the telomere shortening rate of 2BS cells, which is mainly due to the biological properties of the compounds including the reduction of DNA damage and the improvement of DNA repair ability. In addition, the slow down of telomere shortening rate, the reduction of DNA damage, and the improvement of DNA repair ability induced by HDTIC may be responsible for their delay of replicative senescence.
基金：
grants from the National Natural Science Foundation of China, No. 30672469; National Natural Science Foundation of Beijing, No. 7062030;
the National Basic Research Programs of China, No. 2007CB507400; and Beijing Commission on Science and Technology D07050701130000.
第一作者机构：[1]Aging Research Center, Health and Science School of Peking University, Beijing, People’s Republic of China.[2]Medical Test Department, Xuanwu Hospital of Capital Medical University, Beijing, People’s Republic of China.
通讯作者：
通讯机构：[*]Aging Research Center, Health and Science School of Peking University No. 38, Xueyuan Road Beijing 100083 People’s Republic of China.
推荐引用方式(GB/T 7714)：
Peichang Wang ,Zongyu Zhang ,Ying Sun ,et al.The Two Isomers of HDTIC Compounds from Astragali Radix Slow Down Telomere Shortening Rate via Attenuating Oxidative Stress and Increasing DNA Repair Ability in Human Fetal Lung Diploid Fibroblast Cells[J].DNA AND CELL BIOLOGY.2010,29(1):33-39.doi:10.1089/dna.2009.0932.
APA：
Peichang Wang,,Zongyu Zhang,,Ying Sun,,Xinwen Liu,&Tanjun Tong.(2010).The Two Isomers of HDTIC Compounds from Astragali Radix Slow Down Telomere Shortening Rate via Attenuating Oxidative Stress and Increasing DNA Repair Ability in Human Fetal Lung Diploid Fibroblast Cells.DNA AND CELL BIOLOGY,29,(1)
MLA：
Peichang Wang,,et al."The Two Isomers of HDTIC Compounds from Astragali Radix Slow Down Telomere Shortening Rate via Attenuating Oxidative Stress and Increasing DNA Repair Ability in Human Fetal Lung Diploid Fibroblast Cells".DNA AND CELL BIOLOGY 29..1(2010):33-39
