机构：[1]Departments of Interventional Radiology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China[2]Departments of Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China[3]Departments of Clinical Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China[4]The Molecular Laboratory Center, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China[5]College of Pharmaceutical Science, Soochow University, Suzhou 215000, Jiangsu, China[6]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu, China
出处：
ISSN：
摘要：
Both of chronic inflammation and abnormal immune in inflammatory bowel disease can induce colon cancer. Previous research showed that cell apoptosis and necrosis become the main source of circulating DNA in the peripheral blood during tumorigenesis that reduced along with methylation degree. However, its role in the process of colitis transforming to colon cancer is not clarified. Drinking 3% DSS was used to establish colitis model, while 3% dextran sodium sulfate (DSS) combined with azo oxidation methane (AOM) intraperitoneal injection was applied to establish colitis related colon cancer model. Circulating DNA and its methylation level in peripheral blood were tested. Morphology observation, HE staining, and p53 and β-catenin expression detection confirmed that drinking 3% DSS and 3% DSS combined with AOM intraperitoneal injection can successfully establish colitis and colitis associated colorectal cancer models. Circulating DNA level in colitis and colon cancer mice increased by gradient compared with control, while significant difference was observed between each other. Circulating DNA methylation level decreased obviously in colitis and colon cancer, and significant difference was observed between each other. Abnormal protein expression, circulating DNA and its methylation level in ulcerative colitis associated colorectal tissues change in gradient, suggesting that circulating DNA and its methylation level can be treated as new markers for colitis cancer transformation that has certain significance to explore the mechanism of human ulcerative colitis canceration.
基金：
This project was partially supported by Chinese
Natural Science Foundation (81172348); The
project of Jiangsu province health department
(H201313); The project of Jiangsuprovince
science and technology department (BL2013-
016); The second affiliated hospital of Soochow
university preponderant clinic discipline
group project funding and The Suzhou Science
and Technology Development Program (SZS-
201509).
第一作者机构：[1]Departments of Interventional Radiology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxian Rd, Suzhou 215000, Jiangsu, China.[*2]Department of Interventional Radiology, The Second Affiliated Hospital of Soochow University, 1055 Sanxian Rd, Suzhou 215000, Jiangsu, China.
推荐引用方式(GB/T 7714)：
Xuming Bai,Yaqun Zhu,Wangyang Pu,et al.Circulating DNA and its methylation level in inflammatory bowel disease and related colon cancer.[J].International journal of clinical and experimental pathology.2015,8(10):13764-9.
APA：
Xuming Bai,Yaqun Zhu,Wangyang Pu,Li Xiao,Kai Li...&Yong Jin.(2015).Circulating DNA and its methylation level in inflammatory bowel disease and related colon cancer..International journal of clinical and experimental pathology,8,(10)
MLA：
Xuming Bai,et al."Circulating DNA and its methylation level in inflammatory bowel disease and related colon cancer.".International journal of clinical and experimental pathology 8..10(2015):13764-9
