机构：[1]Laboratory of Nutrition, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, People’s Republic of China临床科室科研平台职能科室临床流行病与循证医学中心临床营养科儿科研究所首都医科大学附属北京儿童医院[2]Department of Child Health Care, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, People’s Republic of China职能科室临床流行病与循证医学中心首都医科大学附属北京儿童医院
Specific adipokines, such as adiponectin and resistin, are secreted from adipose tissue and are associated with the development of obesity. Supplementation of dietary SCFA can prevent and reverse high-fat-diet (HFD)-induced obesity. However, it is not clear whether SCFA ameliorate abnormal expression of adiponectin and resistin in the obese state. The aim of this study was to investigate the effects of SCFA on adiponectin and resistin's expressions in diet-induced obese mice, as well as the potential mechanisms associated with DNA methylation. C57BL/6J male mice were fed for 16 weeks with five types of HFD (34.9 % fat by wt., 60% kJ) - a control HFD and four HFD with acetate (HFD-A), propionate (HFD-P), butyrate (HFD-B) and their admixture (HFD-SCFA). Meanwhile, a low-fat diet (4.3% fat by wt., 10 % kJ) was used as the control group. The reduced mRNA levels of adiponectin and resistin in the adipose tissue of the HFD-fed mice were significantly reversed by dietary supplementation of acetate, propionate, butyrate or their admixture to the. HFD. Moreover, the expressional changes of adiponectin and resistin induced by SCFA were associated with alterations in DNA methylation at their promoters, which was mediated by reducing the expressions of enzyme-catalysed DNA methyltransferase (DNMT1, 3a, 3b) and the methyl-CpG-binding domain protein 2 (MBD2) and suppressing the binding of these enzymes to the promoters of adiponectin and resistin. Our results indicate that SCFA may correct aberrant expressions of adiponectin and resistin in obesity by epigenetic regulation.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81670775]; Research Funds of Profession Quota Budjet from Beijing Municipal Science and Technology Commission [2016-bjsekyjs-2]
第一作者机构：[1]Laboratory of Nutrition, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, People’s Republic of China[2]Department of Child Health Care, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Laboratory of Nutrition, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, People’s Republic of China
推荐引用方式(GB/T 7714)：
Lu Yuanyuan,Fan Chaonan,Liang Aimin,et al.Effects of SCFA on the DNA methylation pattern of adiponectin and resistin in high-fat-diet-induced obese male mice[J].BRITISH JOURNAL OF NUTRITION.2018,120(4):385-392.doi:10.1017/S0007114518001526.
APA：
Lu, Yuanyuan,Fan, Chaonan,Liang, Aimin,Fan, Xiuqin,Wang, Rui...&Qi, Kemin.(2018).Effects of SCFA on the DNA methylation pattern of adiponectin and resistin in high-fat-diet-induced obese male mice.BRITISH JOURNAL OF NUTRITION,120,(4)
MLA：
Lu, Yuanyuan,et al."Effects of SCFA on the DNA methylation pattern of adiponectin and resistin in high-fat-diet-induced obese male mice".BRITISH JOURNAL OF NUTRITION 120..4(2018):385-392
