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An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells (vol 7, 2320, 2018)

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机构: [1]Fudan Univ, Shanghai Med Coll, Inst Biomed Sci, Shanghai 200032, Peoples R China; [2]Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200438, Peoples R China; [3]Fudan Univ, Sch Life Sci, MOE Key Lab Contemporary Anthropol, Shanghai 200438, Peoples R China; [4]Fudan Univ, Inst Reprod & Dev Obstet & Gynecol Hosp, Key Lab Reprod Regulat NPFPC SIPPR, Shanghai 200011, Peoples R China; [5]Capital Med Univ, Beijing Insitute Heart Lung & Blood Vessel Dis, Beijing Anzhen Hosp, Beijing 100029, Peoples R China; [6]Southeast Univ, Inst Life Sci, Coinnovat Ctr Neuro Regenerat, Key Lab Dev Genes & Human Dis,Minist Educ, Nanjing 210096, Jiangsu, Peoples R China; [7]Nantong Univ, Coinnovat Ctr Neuroregenerat, Jiangsu Key Lab Neuroregenerat, Nantong 226001, Peoples R China; [8]Soochow Univ, Affiliated Hosp 1, Inst Cardiovasc Sci, Suzhou 215007, Peoples R China; [9]Soochow Univ, Affiliated Hosp 1, Dept Cardiovasc Surg, Suzhou 215007, Peoples R China; [10]Fudan Univ, Childrens Hosp, 399 Wanyuan Rd, Shanghai 201102, Peoples R China
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The authors felt they should cite the following article by Ohashi M, et al. (2015), which is included below as Reference 1. Specifically, in the Introduction the sentence, “Recently, Li et al. have achieved high efficiency (8–76%) of genome editing by using an episomal vector to express Cas9 and gRNA23.” should read: “Recently, two groups have achieved high efficiency of genome editing by using an episomal vector to express Cas9 and gRNA in both human somatic cells and mouse iPSCs23, 1.” In addition, in the Discussion, the sentence: “When we were preparing the manuscript, another group reported that the episomal CRISPR/Cas9 system could work efficiently in HeLa cells and mouse iPSCs23.” should read: “Two groups have reported that the episomal CRISPR/Cas9 system could work efficiently in both human somatic cells and mouse iPSCs23, 1.” © 2018, The Author(s).

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大类 | 3 区 综合性期刊
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第一作者机构: [1]Fudan Univ, Shanghai Med Coll, Inst Biomed Sci, Shanghai 200032, Peoples R China;
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通讯机构: [2]Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200438, Peoples R China; [3]Fudan Univ, Sch Life Sci, MOE Key Lab Contemporary Anthropol, Shanghai 200438, Peoples R China; [4]Fudan Univ, Inst Reprod & Dev Obstet & Gynecol Hosp, Key Lab Reprod Regulat NPFPC SIPPR, Shanghai 200011, Peoples R China; [10]Fudan Univ, Childrens Hosp, 399 Wanyuan Rd, Shanghai 201102, Peoples R China
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