机构：[1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China神经科系统神经外科首都医科大学宣武医院[2]Spine Center, China International Neuroscience Institute (CHINA-INI), Beijing, China[3]Research Center of Spine and Spinal Cord, Beijing Institute of Brain Disorders, Capital Medical University, Beijing, China[4]National Center for Neurological Disorders, Xuanwu Hospital, Beijing, China首都医科大学宣武医院[5]Center for Experimental Animals, Xuanwu Hospital, Capital Medical University, Beijing, China首都医科大学宣武医院[6]Medical Isotopes Research Center and Department of Radiation Medicine, State Key Laboratory of Natural and Biomimetic Drugs, School of Basic Medical Sciences, Peking University, Beijing, China
Objective: Traditional morphological imaging of intervertebral disc herniation (IVDH) is challenging in early disease diagnosis. Aiming at the early diagnosis of IVD by non-invasive molecular imaging targeting of integrin alpha 5 beta 1, we performed novel imaging in rats with acute IVDH for the first time.& nbsp;Methods: Animal models were prepared by conducting an established needle puncture procedure through the normal intervertebral disc (IVD). The disc-injured rats underwent SPECT/CT imaging of the Tc-99m-3PisoDGR2 peptide at 1 day to 2 months postinjury. The expression change of integrin alpha 5 beta 1 was determined by anti-integrin alpha 5 and anti-integrin alpha 5 beta 1 immunohistochemistry (IHC). Magnetic resonance imaging (MRI) was performed for comparison during disease progression. The morphological changes of the disc were determined by safranin-O staining.& nbsp;Results: Rats with acute IVDH showed gradually increased disc uptake of Tc-99m-3PisoDGR2 from 1 to 7 days posttreatment, which was a significantly higher level than that of the normal disks in degenerative diseases. IHC results showed the expression of integrin alpha 5 beta 1 on the surface of annulus fibrosus (AF) cells and nucleus pulposus (NP) cells, which agreed with the uptake data. MRI showed a progressively decreased T2 density and MRI index throughout the investigation. Hematoxylin and eosin (HE) staining and safranin-O staining revealed a disorganized structure of the IVD as well as loss of proteoglycans after puncture.& nbsp;Conclusions: The present study demonstrated a good correlation between integrin alpha 5 beta 1 expression and acute disc herniation. The SPECT/CT imaging of Tc-99m-3PisoDGR2 targeting integrin alpha 5 beta 1 may diagnose IVDH in an acute phase for early disease management.
基金：
Beijing Municipal Science and Technology Commission [Z191199996619048]; Beijing Municipal Commission of Education [KZ202010025043, 1192070315]
第一作者机构：[1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China[2]Spine Center, China International Neuroscience Institute (CHINA-INI), Beijing, China[3]Research Center of Spine and Spinal Cord, Beijing Institute of Brain Disorders, Capital Medical University, Beijing, China[4]National Center for Neurological Disorders, Xuanwu Hospital, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China[2]Spine Center, China International Neuroscience Institute (CHINA-INI), Beijing, China[3]Research Center of Spine and Spinal Cord, Beijing Institute of Brain Disorders, Capital Medical University, Beijing, China[4]National Center for Neurological Disorders, Xuanwu Hospital, Beijing, China
推荐引用方式(GB/T 7714)：
Guan Jian,Yuan Chenghua,Tian Xin,et al.SPECT Imaging of Acute Disc Herniation by Targeting Integrin alpha 5 beta 1 in Rat Models[J].FRONTIERS IN NEUROLOGY.2022,13:doi:10.3389/fneur.2022.782967.
APA：
Guan, Jian,Yuan, Chenghua,Tian, Xin,Cheng, Lei,Gao, Hannan...&Jian, Fengzeng.(2022).SPECT Imaging of Acute Disc Herniation by Targeting Integrin alpha 5 beta 1 in Rat Models.FRONTIERS IN NEUROLOGY,13,
MLA：
Guan, Jian,et al."SPECT Imaging of Acute Disc Herniation by Targeting Integrin alpha 5 beta 1 in Rat Models".FRONTIERS IN NEUROLOGY 13.(2022)
