机构：[1]Department of Vascular Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Department of Vascular Surgery, Xuan Wu Hospital, Capital Medical University, Beijing, China.外科系统血管外科首都医科大学宣武医院
To develop small-diameter (<6 mm) scaffolds capable of accelerating rapid endothelialization and improving long-term patency rate, we created acellular vascular scaffolds preloaded with heparin and hepatocyte growth factor (HGF). Heparin was conjugated to suppress thrombogenic responses, and HGF was immobilized to induce endothelial cells (ECs) proliferation and migration. The scaffolds immobilized with heparin exhibited highly effective localization and sustained release of HGF for 30 days <i>in vitro</i>. We implanted this modified scaffold into the carotid artery of a rabbit model to investigate the efficacy <i>in vivo</i>. The acellular vascular scaffold with heparin only was used as control. After transplantation, the patency of this modified scaffold was 91.67% at 1, 3, 6, and 12 months, while the patency rate in the group with grafted heparin only was 83.33% at 1, 3, 6, and 12 months. This modified scaffold significantly stimulated ECs proliferation and the endothelium aligned in the direction of flow after 12 months. In addition, intimal hyperplasia was significantly reduced in the grafts coated with HGF compared with the control grafts. The small-diameter vascular grafts with an inner diameter of 2.5 mm preloaded with heparin and HGF may be a substitute for autologous blood vessels in clinic.
基金：
National Key Research and Development
Program of China (Grant 2017YFC1104100) and the Capital Health
Research and Development of Special, Beijing, China (Grant
2016-1-2012).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|3 区医学
小类|3 区医学：研究与实验3 区移植4 区细胞与组织工程
最新[2023]版：
大类|4 区医学
小类|4 区细胞与组织工程4 区医学：研究与实验4 区移植
JCR分区：
出版当年[2020]版：
Q2TRANSPLANTATIONQ2MEDICINE, RESEARCH & EXPERIMENTALQ3CELL & TISSUE ENGINEERING
最新[2023]版：
Q2MEDICINE, RESEARCH & EXPERIMENTALQ2TRANSPLANTATIONQ3CELL & TISSUE ENGINEERING
第一作者机构：[1]Department of Vascular Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
通讯作者：
通讯机构：[1]Department of Vascular Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.[2]Department of Vascular Surgery, Xuan Wu Hospital, Capital Medical University, Beijing, China.[*1]Department of Vascular Surgery, Beijing Tongren Hospital, Capital Medical University, No.1 Dong Jiao Min Xiang Street, Dongcheng District, Beijing 100730, China[*2]Department of Vascular Surgery, Xuan Wu Hospital, Capital Medical University, No. 45, Changchun Street, Xicheng District, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Cai Zhiwen,Tan Zhengli,Tian Ran,et al.Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal Hyperplasia[J].CELL TRANSPLANTATION.2022,31:doi:10.1177/09636897221134541.
APA：
Cai Zhiwen,Tan Zhengli,Tian Ran,Chen Xin,Miao Peng...&Gu Yongquan.(2022).Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal Hyperplasia.CELL TRANSPLANTATION,31,
MLA：
Cai Zhiwen,et al."Acellular Vascular Scaffolds Preloaded With Heparin and Hepatocyte Growth Factor for Small-Diameter Vascular Grafts Might Inhibit Intimal Hyperplasia".CELL TRANSPLANTATION 31.(2022)
