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METTL3 Modulates TXNIP Expression to Affect the Activation of NLRP3 Inflammasome in Hepatic Cells Under Oxygen-Glucose Deprivation/Reperfusion Injury

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机构： [1]Anesthesia Department, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, Xi'an, Shaanxi Province, 710004, China. [2]Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing, China. [3]Department of General Practice, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

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关键词： Hepatic ischemia/reperfusion injury m6A modification METTL3 TXNIP NLRP3 inflammasome

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Hepatic ischemia-reperfusion (I/R) injury is still a major risk factor and unsolved problem in hepatic surgery. Methyltransferase-like 3 (METTL3), an important m6A-modified methylase, regulates inflammation and cellular stress response. In this study, we demonstrated the special role of METTL3 and its underlying mechanism in hepatic I/R injury. In the mouse model of hepatic I/R and in the oxygen-glucose deprivation and reoxygenation (OGD/R)-induced AML12 and NCTC 1469 cells, the expression of METTL3 was significantly upregulated. Inhibition of METTL3 in OGD/R-induced AML12 and NCTC 1469 cells both increased the cell viability, declined the cell apoptosis, and decreased the reactive oxygen species (ROS) and the release levels of interleukin-1β (IL-1β) and interleukin-18 (IL-18), diminishing NLRP3 and Caspase1-p20 expressions. Moreover, METTL3 positively modulated TXNIP expression in an m6A manner. TXNIP overexpression reversed the effects of METTL3 knockdown on OGD/R-induced injury in AML12 cells. Furthermore, inhibition of NLRP3 inflammasome activity contributed to the protective effects of TXNIP knockdown in OGD/R-induced AML12 cells. In conclusion, METTL3 knockdown alleviated OGD/R-induced hepatocyte injury, and the specific mechanism was associated with the inhibition of NLRP3 inflammasome activation, which was attributed to the reduction of TXNIP in an m6A-dependent manner.© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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出版当年[2023]版：

大类 | 2 区医学

小类 | 3 区细胞生物学 3 区免疫学

最新[2023]版：

大类 | 2 区医学

小类 | 3 区细胞生物学 3 区免疫学

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出版当年[2022]版：

Q2 CELL BIOLOGY Q2 IMMUNOLOGY

最新[2023]版：

Q2 CELL BIOLOGY Q2 IMMUNOLOGY

影响因子： 4.5 最新[2023版] 4.6 最新五年平均 5.1 出版当年[2022版] 4.8 出版当年五年平均 4.657 出版前一年[2021版] 4.5 出版后一年[2023版]

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第一作者机构： [1]Anesthesia Department, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, Xi'an, Shaanxi Province, 710004, China.

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