机构：[1]Center for Translational Medicine, The Third People's Hospital of Longgang District, Shenzhen, PRC.[2]Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou, PRC.[3]Neuroscience Institute, Baotou Medical College, Inner Mongolia, PRC.内蒙古科技大学包头医学院[4]Department of Cardiology, The Third People's Hospital of Longgang District, Shenzhen, PRC.深圳市康宁医院深圳医学信息中心[5]Department of Cardiology, The People's Hospital of Longgang District, Shenzhen, PRC.深圳市康宁医院深圳医学信息中心[6]Zhongshan School of medicine, Sun Yat-sen University, Guangzhou, PRC.[7]Department of Oral and Maxillofacial Surgery, University of California San Francisco, San Francisco, USA.[8]Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia, PRC.内蒙古科技大学包头医学院[9]Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, Beijing, PRC.科技平台低氧适应转化医学北京市重点实验室首都医科大学宣武医院[10]Joint Laboratory of South China Hospital Affiliated to Shenzhen University and Third People's Hospital of Longgang District, Shenzhen University, Shenzhen, PRC.
Wang L, Fu G, Han R, Fan P, Yang J, Gong K, Zhao Z, Zhang C, Sun K, Shao GMALAT1 and NEAT1 Are Neuroprotective during Hypoxic Preconditioning in the Mouse Hippocampus Possibly by Regulation of NR2B High Alt Med Biol. 00:000-000, 2024. Background: The regulation of noncoding ribonucleic acid (ncRNA) has been shown to be involved in cellular and molecular responses to hypoxic preconditioning (HPC), a situation created by the induction of sublethal hypoxia in the brain. The ncRNAs metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and nuclear paraspeckle assembly transcript 1 (NEAT1) are abundantly expressed in the brain, where they regulate the expression of various genes in nerve cells. However, the exact roles of MALAT1 and NEAT1 in HPC are not fully understood. Methods: A mouse model of acute repeated hypoxia was used as a model of HPC, and MALAT1 and NEAT1 levels in the hippocampus were measured using real-time polymerase chain reaction (PCR). The mRNA and protein levels of N-methyl-d-aspartate receptor subunit 2 B (NR2B) in the mouse hippocampus were measured using real-time PCR and western blotting, respectively. HT22 cells knocked-down for MALAT1 and NEAT1 were used for in vitro testing. Expression of NR2B, which is involved in nerve cell injury under ischemic and hypoxic conditions, was also evaluated. The levels of spectrin and cleaved caspase-3 in MALAT1 and NEAT1 knockdown HT22 cells under oxygen glucose deprivation/reperfusion (OGD/R) were determined by western blotting. Results: HPC increased the expression of MALAT1 and NEAT1 and decreased the expression of NR2B mRNA in the mouse hippocampus (p < 0.05). Knockdown of MALAT1 and NEAT1 increased both NR2B mRNA and protein levels nearly twofold and caused damage under OGD/R conditions in HT22 cells (p < 0.05). Conclusion: MALAT1 and NEAT1 exert neuroprotective effects by influencing the expression of NR2B.
基金：
The National Natural Science Foundation of China
(Nos.81660307, 82060337), Funds for Shenzhen Longgang
District Economic and Technological Development Special Fund
Medical, Health Technology Plan Project (Nos.LGKCYLWS2021000033, LGKCYLWS2023025), and Shenzhen Science
and Technology Plan Project (Nos.JCYJ20220531092412028,JCYJ20230807121306012)
第一作者机构：[1]Center for Translational Medicine, The Third People's Hospital of Longgang District, Shenzhen, PRC.[2]Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou, PRC.[3]Neuroscience Institute, Baotou Medical College, Inner Mongolia, PRC.
共同第一作者：
通讯作者：
通讯机构：[1]Center for Translational Medicine, The Third People's Hospital of Longgang District, Shenzhen, PRC.[2]Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou, PRC.[3]Neuroscience Institute, Baotou Medical College, Inner Mongolia, PRC.[8]Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia, PRC.[9]Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, Beijing, PRC.[10]Joint Laboratory of South China Hospital Affiliated to Shenzhen University and Third People's Hospital of Longgang District, Shenzhen University, Shenzhen, PRC.[*1]Center for Translational Medicine The Third People’s Hospital of Longgang District Shenzhen PRC[*2]Department of Neurosurgery The First Affiliated Hospital of Baotou Medical College Inner Mongolia PRC
推荐引用方式(GB/T 7714)：
Wang Liping,Fu Gang,Han Ruijuan,et al.MALAT1 and NEAT1 Are Neuroprotective During Hypoxic Preconditioning in the Mouse Hippocampus Possibly by Regulation of NR2B[J].High Altitude Medicine & Biology.2024,25(4):285-294.doi:10.1089/ham.2023.0135.
APA：
Wang Liping,Fu Gang,Han Ruijuan,Fan Peijia,Yang Jing...&Shao Guo.(2024).MALAT1 and NEAT1 Are Neuroprotective During Hypoxic Preconditioning in the Mouse Hippocampus Possibly by Regulation of NR2B.High Altitude Medicine & Biology,25,(4)
MLA：
Wang Liping,et al."MALAT1 and NEAT1 Are Neuroprotective During Hypoxic Preconditioning in the Mouse Hippocampus Possibly by Regulation of NR2B".High Altitude Medicine & Biology 25..4(2024):285-294
