机构：[1]The MOE Key Laboratory for Standardisation of Chinese Medicines and the Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China[2]Shaanxi Academy of Traditional Chinese Medicine, Xi'an, China[3]Clinical Pharmacology Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China[4]Xi'an Institute for Food and Drug Control, Xi'an, China
Introduction Erycibe obtusifolia and E. schmidtii are widely used in traditional Chinese medicine (TCM) to treat joint pain and rheumatoid arthritis. With the reduction of wild E. obtusifolia and E. schmidtii resources, Porana sinensis has been widely used as a substitute. However, few studies have been conducted on the chemical composition and quality control of P. sinensis. Objective To clarify the chemical composition and improve the quality control of P. sinensis. Methodology We developed an ultra-high performance liquid chromatography electrospray ionisation Q-Exactive Focus tandem mass spectrometry (UHPLC-ESI-Q-Exactive Focus-MS/MS) method to characterise the chemical constituents of P. sinensis. A strategy based on a combination of high-performance thin-layer chromatography (HPTLC) and direct analysis in real-time (DART) ion source was proposed for the identification of alkaloid components in P. sinensis. Thin-layer chromatography (TLC) autography for 2,2 '-diphenyl-1-picrylhydrazyl free radical (DPPH) and TLC bioautography for xanthine oxidase were used to rapidly screen marker compounds for high-performance liquid chromatography (HPLC) determination of P. sinensis. Based on the selected marker compounds, a HPLC method for the quantitative determination of eight marker compounds in P. sinensis was developed. Results Eighteen compounds in P. sinensis were identified by UHPLC-Q-Exactive MS. Taken together with the results of TLC autography and TLC bioautography, eight compounds were chosen as marker compounds for HPLC determination of P. sinensis. The alkaloid components in P. sinensis were identified as Baogongteng A and Baogongteng C by DART-MS. Conclusion We systematically clarified the chemical composition of P. sinensis for the first time, and potentially improved its quality control. These results should promote the application of P. sinensis as a new resource for Caulis Erycibes.
基金：
Project of Shaanxi Provincial Youth Science
and Technology Star Plan, Grant/Award
Number: 2018KJXX‐073; National Natural
Science Foundation of China (NSFC), Grant/
Award Number: 81603264
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|3 区生化研究方法3 区分析化学3 区植物科学
最新[2023]版：
大类|3 区生物学
小类|3 区生化研究方法3 区分析化学3 区植物科学
JCR分区：
出版当年[2017]版：
Q2PLANT SCIENCESQ2CHEMISTRY, ANALYTICALQ3BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2CHEMISTRY, ANALYTICALQ2PLANT SCIENCES
第一作者机构：[1]The MOE Key Laboratory for Standardisation of Chinese Medicines and the Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China[2]Shaanxi Academy of Traditional Chinese Medicine, Xi'an, China
共同第一作者：
通讯作者：
通讯机构：[*1]Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China.[*2]Shaanxi Academy of Traditional Chinese Medicine, Xi'an, Shaanxi 710003, China.
推荐引用方式(GB/T 7714)：
Zhiyong Chen,Mengmeng Wang,Yuanyuan Yang,et al.Qualitative and quantitative analysis of Porana sinensis Hemsl by UHPLC-Q-Exactive MS, TLC autographic method and DART-MS[J].PHYTOCHEMICAL ANALYSIS.2019,30(3):311-319.doi:10.1002/pca.2814.
APA：
Zhiyong Chen,Mengmeng Wang,Yuanyuan Yang,Xia Du,Zijia Zhang&Ye Li.(2019).Qualitative and quantitative analysis of Porana sinensis Hemsl by UHPLC-Q-Exactive MS, TLC autographic method and DART-MS.PHYTOCHEMICAL ANALYSIS,30,(3)
MLA：
Zhiyong Chen,et al."Qualitative and quantitative analysis of Porana sinensis Hemsl by UHPLC-Q-Exactive MS, TLC autographic method and DART-MS".PHYTOCHEMICAL ANALYSIS 30..3(2019):311-319
