机构：[1]Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China[2]Department of Pathology, WuXi Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, China[3]Department of General Surgery, The People’s Hospital of Gaomi, Gaomi, China
OBJECTIVE: Cell autophagy reduces the sensitivity of cancer cells to therapeutic reagents in various types of human cancer. Therefore, the aim of our study was to use human colorectal cancer HCT116 cells to explore whether inhibition of autophagy by 3-Methyladenine (3-MA, an autophagy inhibitor) is able to enhance hypoxia-induced apoptosis in vitro. MATERIALS AND METHODS: HCT116 cells were treated with 3-MA. hypoxia. or 3-MA plus hypoxia. and the autophagy. apoptosis and proliferation of the HCT116 cells were investigated. Western blot analysis was used to detect autophagy specificity protein microtubule-associated protein light chain 3 (LC3) expression. Effects on apoptosis were evaluated by using flow cytometry (JC-1 staining to measure mitochondrial membrane potential) and annexin V-propidium iodide (PI) staining. RESULTS: The results showed that the treatment of HCT116 cells in vitro with hypoxia alone increased autophagy as well as apoptosis, whereas combination treatment with 3-MA and hypoxia markedly inhibited hypoxia-induced autophagy. but increased hypoxia-induced cell apoptosis. CONCLUSIONS: Autophagy might play a role as a self-defense mechanism in hypoxia-treated colon cancer cells, and its inhibition could be a promising strategy for the adjuvant chemotherapy of colon cancer.
基金：
National Natural Science Foundation of China [81672970]; Projects of Suzhou Technology Bureau [SYS201552, SZS201618, SS201753]; focus of Clinical Disease Treatment Technology Special Funds of Suzhou City [LCZX201505]; Second Affiliated Hospital of Soochow University Preponderant Clinic Discipline Group Project Funding
第一作者机构：[1]Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China[2]Department of Pathology, WuXi Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China
推荐引用方式(GB/T 7714)：
Dong Y.,Wu Y.,Zhao G. -L.,et al.Inhibition of autophagy by 3-MA promotes hypoxia-induced apoptosis in human colorectal cancer cells[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2019,23(3):1047-1054.doi:10.26355/eurrev_201902_16992.
APA：
Dong, Y.,Wu, Y.,Zhao, G. -L.,Ye, Z. -Y.,Xing, C. -G.&Yang, X. -D..(2019).Inhibition of autophagy by 3-MA promotes hypoxia-induced apoptosis in human colorectal cancer cells.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,23,(3)
MLA：
Dong, Y.,et al."Inhibition of autophagy by 3-MA promotes hypoxia-induced apoptosis in human colorectal cancer cells".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 23..3(2019):1047-1054
