机构:[1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Department of General Surgery, The First People’s Hospital of Taicang City, Taicang Affiliated Hospital of Soochow University, Suzhou 215400, China[3]School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China[4]Oncology Department, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Autophagy is a double-edged sword in tumor development. Recent studies have found that miRNAs have an inhibitory effect on the regulation of autophagy. It has been reported that miR-31 plays an important role in the development of colorectal cancer. However, what role miR-31 plays in colorectal cancer-associated fibroblasts (CAFs) has not been determined. In this study, we confirmed that the expression of miR-31 in CAFs was higher than in normal colorectal fibroblasts (NFs). We also found that treatment of CAFs with miR-31 mimic inhibited the expression of the autophagy-related genes Beclin-1, ATG, DRAM and LC3. In addition, we found up-regulation of miR-31 significantly affected colorectal cancer cell behaviors, including proliferation, invasion and apoptosis. Also, up-regulation of miR-31 in CAF could increase the radiosensitivity of colorectal cancer cells co-cultured with CAF. In summary, miR-31 can inhibit autophagy in colorectal CAFs, affect colorectal cancer development, and increase the radiosensitivity of colorectal cancer cells co-cultured with CAF. We hypothesize that miR-31 may become a new target of treatments for colorectal cancer.
基金:
This work was partially supported by the National Natural Science Foundation of China (Grant No: 81672970, 81301933 , 81472917and 81572345), Health Research Projects in Jiangsu Province (H201313), the projects of Suzhou Technology Bureau (SYSD2013090, SYS201552, SYSD2015034) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Suzhou Science and Education health youth projects (kjxw2015050), Taicang Municipal Science and Technology Bureau of Basic Research Program (TC2015YYYL01), the focus of clinical disease treatment technology special funds of Suzhou city (LCZX201505), and the second affiliated hospital of Soochow university preponderant clinic discipline group project funding.
第一作者机构:[1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
共同第一作者:
通讯作者:
通讯机构:[1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[3]School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China
推荐引用方式(GB/T 7714):
Xiaodong Yang,Xiaohui Xu,Junjia Zhu,et al.miR-31 affects colorectal cancer cells by inhibiting autophagy in cancer-associated fibroblasts[J].ONCOTARGET.2016,7(48):79617-79628.doi:10.18632/oncotarget.12873.
APA:
Xiaodong Yang,Xiaohui Xu,Junjia Zhu,Shuyu Zhang,Yong Wu...&Wei Peng.(2016).miR-31 affects colorectal cancer cells by inhibiting autophagy in cancer-associated fibroblasts.ONCOTARGET,7,(48)
MLA:
Xiaodong Yang,et al."miR-31 affects colorectal cancer cells by inhibiting autophagy in cancer-associated fibroblasts".ONCOTARGET 7..48(2016):79617-79628