机构：[1]Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou City, PR China[2]Faculty of Health Sciences, College of Pharmacy, University of Manitoba, Manitoba, Winnipeg R3E 3P4, Canada[3]Institute of Neuroscience, Soochow University, Suzhou City, PR China[4]Department of Orthopedics, Clinical Medical College, Yangzhou University, Yangzhou City, PR China[5]Department of Orthopedics, Northern Jiangsu People’s Hospital, Yangzhou City, PR China
Aim: Roles of DNA 5-hydroxymethylcytosine (5hmC) in myelin repair were investigated in an experimental autoimmune encephalomyelitis (EAE) mouse model via its regulation on BDNF. Methods: DNA 5hmC level and its limiting enzymes were detected in EAE mice. Results: Global 5hmC modification, Tet1 and Tet2 significantly decreased in the spinal cord tissues of EAE mice. BDNF protein and mRNA decreased and were highly associated with BDNF 5hmC. Vitamin C, a Tet co-factor, increased global DNA 5hmC and reduced the neurological deficits at least by increasing BDNF5hmC modification and protein levels. Conclusion: Tet protein-mediated 5hmC modifications represent a critical target involved in EAE-induced myelin damage. Targeting epigenetic modification may be a therapeutic strategy for multiple sclerosis.
基金：
National Key R&D Program of China [2017YFE0103700]; National Natural Science Foundation of China [81601154]; Natural Science Foundation of Jiangsu Province [BK20141281]; Special Foundation Project on the Prospective Study of Social Development in Jiangsu Province [BE2013911]; Jiangsu Six Categories of Talent Summit Fund [WSW-133]; Social Development of Science and Technology Research Project in Yangzhou [YZ2011082]; Jiangsu Province 333 talent Project [BRA2016159]
第一作者机构：[1]Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou City, PR China[2]Faculty of Health Sciences, College of Pharmacy, University of Manitoba, Manitoba, Winnipeg R3E 3P4, Canada[3]Institute of Neuroscience, Soochow University, Suzhou City, PR China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou City, PR China[2]Faculty of Health Sciences, College of Pharmacy, University of Manitoba, Manitoba, Winnipeg R3E 3P4, Canada[3]Institute of Neuroscience, Soochow University, Suzhou City, PR China[4]Department of Orthopedics, Clinical Medical College, Yangzhou University, Yangzhou City, PR China[5]Department of Orthopedics, Northern Jiangsu People’s Hospital, Yangzhou City, PR China
推荐引用方式(GB/T 7714)：
Tang Yan,Luo Man,Pan Kailing,et al.<bold>DNA hydroxymethylation changes in response to spinal cord damage in a multiple sclerosis mouse model</bold>[J].EPIGENOMICS.2019,11(3):323-335.doi:10.2217/epi-2018-0162.
APA：
Tang, Yan,Luo, Man,Pan, Kailing,Ahmad, Tina,Zhou, Ting...&Wang, Yongxiang.(2019).<bold>DNA hydroxymethylation changes in response to spinal cord damage in a multiple sclerosis mouse model</bold>.EPIGENOMICS,11,(3)
MLA：
Tang, Yan,et al."<bold>DNA hydroxymethylation changes in response to spinal cord damage in a multiple sclerosis mouse model</bold>".EPIGENOMICS 11..3(2019):323-335
