机构：[a]Department of Haematology, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China[b]Department of Cell Biology, School of Biology & Basic Medical Sciences, Soochow University, Suzhou, People’s Republic of China[c]Department of Bioinformatics, School of Biology & Basic Medical Sciences, Soochow University, Suzhou, People’s Republic of China[d]Department of Biochemistry, School of Biology & Basic Medical Sciences, Soochow University, Suzhou, People’s Republic of China[e]Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China
The characteristics of mesenchymal stromal cells (MSCs) which derived from multiple myeloma (MM) patients are typically impaired in osteogenic differentiation. However, the underlying molecular mechanisms need to be further investigated. lncRNAs are emerging as critical regulation molecules in oncogenic pathways. In this study, we identified that bioactive lncRNA HOXC-AS3, which is transcribed in opposite to HOXC10, was presented in MSCs derived from bone marrow (BM) of MM patients (MM-MSCs). HOXC-AS3 was able to interact with HOXC10 at the overlapping parts and this interaction increased HOXC10 stability, then promoted its expression, conferring osteogenesis repression to MM-MSCs. In mouse models, intravenously administered siHOXC-AS3 was proven to be effective in prevention of bone loss, sustained by both anticatabolic activities and bone-forming. These data showed that lncHOXC-AS3 was required for osteogenesis in BM-MSCs by enhancing HOXC10 expression. Our finding thus unveils a novel insight for the potential clinical significance of lncRNA HOXC-AS3 as a therapeutic target for bone disease in MM. Stem Cells 2019;37:247-256
基金：
This research was supported by the Applied Basic Research Programs of Suzhou City (grant no. SYS201546), the Science and Technology Development Project of Suzhou City (grant no. SS201856), National Natural Science Foundation of China (grant nos. 81673448 and 81670191), A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Natural Science Foundation of
Jiangsu Province China (grant nos. BK20161218, BK20161223, and BK20140321).
第一作者机构：[a]Department of Haematology, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Cell Biology, School of Biology & Basic Medical Sciences, Soochow University, Ren Ai Road 199, Suzhou 215123, People’s Republic of China[*2]Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215123, People’s Republic of China
推荐引用方式(GB/T 7714)：
BINGZONG LI ,HUIYING HAN ,SHA SONG ,et al.HOXC10 Regulates Osteogenesis of Mesenchymal Stromal Cells Through Interaction with Its Natural Antisense Transcript lncHOXC-AS3[J].STEM CELLS.2019,37(2):247-256.doi:10.1002/stem.2925.
APA：
BINGZONG LI,,HUIYING HAN,,SHA SONG,,GAO FAN,,HONGXIA XU,...&WENZHUO ZHUANG.(2019).HOXC10 Regulates Osteogenesis of Mesenchymal Stromal Cells Through Interaction with Its Natural Antisense Transcript lncHOXC-AS3.STEM CELLS,37,(2)
MLA：
BINGZONG LI,,et al."HOXC10 Regulates Osteogenesis of Mesenchymal Stromal Cells Through Interaction with Its Natural Antisense Transcript lncHOXC-AS3".STEM CELLS 37..2(2019):247-256
