机构：[1]Cyrus Tang Hematology Center, Soochow University, Suzhou, China[2]Department of Orthopedics, Second Affiliated Hospital of Soochow University, Suzhou, China[3]Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China[4]Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA[5]Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Soochow University, Suzhou, China[6]Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou, China
Mesenchymal stem cells (MSCs) have the potency to differentiate into chondrocytes, osteocytes and adipocytes. Corin is a cardiac protease that activates the natriuretic peptides, thereby regulating blood volume and pressure. In addition to the heart, corin gene upregulation was reported in bone marrow- and adipose tissue-derived MSCs that underwent osteogenic differentiation. To date, the biological significance of corin expression in MSC differentiation remains unknown. In this study we isolated and cultured human bone marrow-derived MSCs that were capable of undergoing chondrogenic, osteogenic and adipogenic lineage differentiation. By reverse transcription polymerase chain reaction (RT-PCR) and immunostaining, we found that corin expression was upregulated when these MSCs underwent chondrogenic, osteogenic and adipogenic differentiation. The upregulation of corin expression was most significant in the cells undergoing chondrogenic lineage differentiation. Silencing corin gene expression by small hairpin RNA in the MSCs inhibited chondrogenic, but not osteogenic and adipogenic, differentiation. These results suggest a novel function of corin in MSC differentiation and chondrocyte development.
基金：
the National Natural Science Foundation of
China, Grant/Award Number: 81370718,
81301844, 81570457, 81671485 and
9163911; the National Basic Research Program
of China, Grant/Award Number:
2015CB943300; Priority Academic Program
Development of Jiangsu Higher Education
Institutes and a grant from the Second Affiliated
Hospital of Soochow University, Grant/
Award Number: SDFEYGJ1603
第一作者机构：[1]Cyrus Tang Hematology Center, Soochow University, Suzhou, China[2]Department of Orthopedics, Second Affiliated Hospital of Soochow University, Suzhou, China
通讯作者：
通讯机构：[*1]Cyrus Tang Hematology Center, Soochow University, Suzhou 215123, China.[*2]Molecular Cardiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
推荐引用方式(GB/T 7714)：
Haibin Zhou,Jinsong Zhu,Meng Liu,et al.Role of the protease corin in chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells[J].JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE.2018,12(4):973-982.doi:10.1002/term.2514.
APA：
Haibin Zhou,Jinsong Zhu,Meng Liu,Qingyu Wu&Ningzheng Dong.(2018).Role of the protease corin in chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells.JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,12,(4)
MLA：
Haibin Zhou,et al."Role of the protease corin in chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 12..4(2018):973-982
