机构：[1]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, No. 1055 Sanxiang Road, Suzhou 215004, China[2]Hematology Center of Cyrus Tang Medical Institute, Soochow University School of Medicine, Suzhou 215123, China[3]Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China[4]Osteoporosis Institute of Soochow University, Suzhou 215004, China
Osteoporosis (OP) is a disease associated with bone loss and microstructure degradation. Recent studies have shown that iron accumulation may be a risk factor for OP. Bone marrow mesenchymal stem cells (MSCs) are multipotent cells and precursors to osteoblasts. MSCs play an important role in OP. Therefore, we evaluated the correlation between MSCs and OP in an environment of iron accumulation. Serum P1NP was decreased in iron accumulation mice. Micro-CT revealed that iron accumulation decreased bone mineral density and spatial structural parameters. Iron accumulation inhibited MSC quantity in bone marrow. However, the iron chelator deferoxamine (DFO) rescued the suppression. Iron accumulation also changed the MSC cell cycle. Iron elevated MSC cell ROS level and NOX4 protein expression. MSC apoptosis was increased, and more caspase3 was cleaved after iron intervention. Our data suggests that iron accumulation inhibits MSC quantity and induces MSC apoptosis. Bone loss from iron accumulation may correlate with the inhibition of MSCs.
基金：
Minsheng Science and Technology Project of Suzhou City [SS201634]; Jiangsu Province's Young Medical Talents Program [QNRC2016880]; the Second Affiliated Hospital of Soochow University Pre-research Foundation [SDFEYQN1608]; Soochow University Youth Teacher Science Fund Project [SDY2011A28]; Natural Science Foundation of China [81502812, 81572179]; Advantage Discipline Groups of the Second Affiliated Hospital of Soochow University [XKQ2015001]; Clinical Medical Center Project of Suzhou City [SZZX201504]; Clinical Medicine Technology Project of Jiangsu Province [BL2014044]
第一作者机构：[1]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, No. 1055 Sanxiang Road, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, No. 1055 Sanxiang Road, Suzhou 215004, China[4]Osteoporosis Institute of Soochow University, Suzhou 215004, China
推荐引用方式(GB/T 7714)：
Ye Yuan,Fei Xu,Yan Cao,et al.Iron Accumulation Leads to Bone Loss by Inducing Mesenchymal Stem Cell Apoptosis Through the Activation of Caspase3[J].BIOLOGICAL TRACE ELEMENT RESEARCH.2019,187(2):434-441.doi:10.1007/s12011-018-1388-9.
APA：
Ye Yuan,Fei Xu,Yan Cao,Li Xu,Chen Yu...&Youjia Xu.(2019).Iron Accumulation Leads to Bone Loss by Inducing Mesenchymal Stem Cell Apoptosis Through the Activation of Caspase3.BIOLOGICAL TRACE ELEMENT RESEARCH,187,(2)
MLA：
Ye Yuan,et al."Iron Accumulation Leads to Bone Loss by Inducing Mesenchymal Stem Cell Apoptosis Through the Activation of Caspase3".BIOLOGICAL TRACE ELEMENT RESEARCH 187..2(2019):434-441
