机构：[1]Department of Neurology, The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University, Wuxi 214000, Jiangsu, China[2]Department of Neurology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Gusu District, Suzhou 215004, Jiangsu, China
Minocycline, an anti-infective agent of a tetracycline derivative, is reported to improve behavioral functional recovery after cerebral ischemia via enhancing the levels of brain-derived neurotrophic factor (BDNF). However, the precise mechanisms that minocycline targets to enhance the expression of BDNF are not fully defined. In the present study, we observed the neuroprotective effect and its potential mechanisms of minocycline using oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells. We found that 50 A mu M minocycline protected against neuronal apoptosis induced by OGD/R injury, with increased expression ratio of Bcl-2/Bax and reduced expression of caspase-3. Interestingly, minocycline resulted in the up-regulation of only BDNF protein, not BDNF mRNA in N2a cells treated with OGD/R. Furthermore, we found that minocycline inhibited OGD/R-induced up-regulation of miR-155 targeted BDNF transcripts. Moreover, miR-155 mimic could partially abolish the neuroprotective effects of minocycline via inhibiting the levels of BDNF protein. These findings suggest that minocycline is neuroprotective against ischemic brain injury through their modulation of miR-155-mediated BDNF repression.
第一作者机构：[1]Department of Neurology, The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University, Wuxi 214000, Jiangsu, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Neurology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Rd, Gusu District, Suzhou 215004, Jiangsu, China
推荐引用方式(GB/T 7714)：
Yunnan Lu,Zhichao Huang,Ye Hua,et al.Minocycline Promotes BDNF Expression of N2a Cells via Inhibition of miR-155-Mediated Repression After Oxygen-Glucose Deprivation and Reoxygenation[J].CELLULAR AND MOLECULAR NEUROBIOLOGY.2018,38(6):1305-1313.doi:10.1007/s10571-018-0599-0.
APA：
Yunnan Lu,Zhichao Huang,Ye Hua&Guodong Xiao.(2018).Minocycline Promotes BDNF Expression of N2a Cells via Inhibition of miR-155-Mediated Repression After Oxygen-Glucose Deprivation and Reoxygenation.CELLULAR AND MOLECULAR NEUROBIOLOGY,38,(6)
MLA：
Yunnan Lu,et al."Minocycline Promotes BDNF Expression of N2a Cells via Inhibition of miR-155-Mediated Repression After Oxygen-Glucose Deprivation and Reoxygenation".CELLULAR AND MOLECULAR NEUROBIOLOGY 38..6(2018):1305-1313
