机构：[1]Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, China,[2]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China,[3]First People’s Hospital of Changshu City, Changshu Hospital Affiliated to Soochow University, Changshu, China,[4]Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China,[5]Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China
beta 1,3-N-acetylglucosaminyltransferase (beta 3GnT8) and beta 3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary beta 1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer. They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine. However, whether beta 3GnT8 and beta 3GnT2 play a role in colorectal cancer and, if so, the underlying mechanisms remain unclear. In our study, we detected the expression of beta 3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices. And beta 3GnT8, CD147, MMP2, and galectin3 were over-expressed in colorectal cancer tissues. We found that overexpression of beta 3GnT8 and beta 3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of beta 3GnT8 and beta 3GnT2 inhibited the invasive activity. Mechanistically, beta 3GnT8 and beta 3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells. Together, these results illustrate that the novel role and the molecular mechanism of beta 3GnT8 and beta 3GnT2 in promotion of colorectal cancer invasion. These results suggest that the potential use of beta 3GnT8 as a tumor target for the therapy of colorectal cancer.
基金：
This study was supported by grants from the National Natural
Science Foundation of China (31400688 and 31170772).
第一作者机构：[1]Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, China,[2]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China,
推荐引用方式(GB/T 7714)：
Zhi Jiang,Huan Zhang,Chunliang Liu,et al.beta 3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis[J].FRONTIERS IN PHYSIOLOGY.2018,9(MAY):588.doi:10.3389/fphys.2018.00588.
APA：
Zhi Jiang,Huan Zhang,Chunliang Liu,Jun Yin,Shan Tong...&Shiliang Wu.(2018).beta 3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis.FRONTIERS IN PHYSIOLOGY,9,(MAY)
MLA：
Zhi Jiang,et al."beta 3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis".FRONTIERS IN PHYSIOLOGY 9..MAY(2018):588
