机构：[1]Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China[2]Department of Neurology, University of Virginia, Charlottesville, VA 22908, USA[3]Clinical Immunology Institute, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China[4]Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou, Jiangsu 215006, P.R. China[5]Jiangsu Key Laboratory of Gastrointestinal tumor Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
Paeoniflorin (PF), a natural compound isolated from Paeoniae radix, has been shown to exert antitumor effects in various types of human cancers including glioma. However, the mechanism of action is not well understood. S-phase kinase-associated protein (Skp)2 functions as an oncogene in many cancers. In the present study, we investigated whether Skp2 mediates the anti-glioma activity of PF. We found that PF inhibited glioma cell proliferation, migration and invasion, and induced G2/M arrest and apoptosis. Skp2 expression was downregulated in glioma cells treated with PF. PF-induced antitumor effects in glioma cells were abolished by Skp2 overexpression but were enhanced by RNA interference of Skp2. Moreover, PF treatment inhibited U87 cell-derived tumor growth in a xenograft mouse model. These results demonstrate that PF exerts its antitumor effects in part by inhibiting Skp2 expression in glioma cells and could be a promising therapeutic agent for glioma therapy.
基金：
the Program of Medical Innovation Team
and Leading Talent of Jiangsu Province, China (no. LJ201150);
the Science and Technology Plan Projects of Jiangsu Province,
China (no. BL2012048) and the National Natural Sciences
Foundation of China (no. 81572480).
第一作者机构：[1]Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China[4]Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou, Jiangsu 215006, P.R. China[5]Jiangsu Key Laboratory of Gastrointestinal tumor Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
通讯作者：
通讯机构：[*1]Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China
推荐引用方式(GB/T 7714)：
Jia Ouyang,Hui Xu1 Ming Li,Xingliang Dai,et al.Paeoniflorin exerts antitumor effects by inactivating S phase kinase-associated protein 2 in glioma cells[J].ONCOLOGY REPORTS.2018,39(3):1052-1062.doi:10.3892/or.2017.6175.
APA：
Jia Ouyang,Hui Xu1, Ming Li,Xingliang Dai,Feng qing Fu,Xueguang Zhang&Qing Lan.(2018).Paeoniflorin exerts antitumor effects by inactivating S phase kinase-associated protein 2 in glioma cells.ONCOLOGY REPORTS,39,(3)
MLA：
Jia Ouyang,et al."Paeoniflorin exerts antitumor effects by inactivating S phase kinase-associated protein 2 in glioma cells".ONCOLOGY REPORTS 39..3(2018):1052-1062
