机构：[1]Department of Endocrinology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China. *Equal contributors
The aim of our study was to investigate the protective effects of Paeoniflorin (PF) against injury induced by AGE-modified bovine serum albumin (AGE-BSA) in human umbilical vein endothelial cells (HUVECs), and to examine the underlying mechanisms of these effects. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to determine cell viability. Protein expression levels were determined by western blotting. For function-blocking experiments, we used small interfering RNA molecules (siRNA) for function-blocking experiments. At 6 h, we found that 100 μg/mL AGE-BSA reduced the viability of HUVECs. However, pretreatment with PF restored cell viability in a dose-dependent manner. AGE-BSA increased the levels of microtubule-associated protein light chain 3-II (LC3-II) and the receptor for advanced glycation end products (RAGE). Expression of p62 protein was also increased, but not at a statistically significant level. Pretreatment with PF further increased levels of LC3-II and RAGE, but reduced the expression of p62. In cells transfected with Atg5 and RAGE siRNA, cell viability and expression of LC3-II decreased in both the AGE-BSA and PF + AGE-BSA treatments. PF can protect HUVECs from AGE-BSA-induced injury by upregulating autophagy and promoting the completion of autophagy flux. RAGE plays an important role in this autophagic protection effect.
基金：
The authors would like to thank Suzhou social development fund (SYS201127), program for revitalizing the youth in science and education of Suzhou (KJXW2011018), and the Jiangsu Province Natural Science Fund of China (SBK-201241136).
第一作者机构：[1]Department of Endocrinology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China
通讯作者：
通讯机构：[*1]Department of Endocrinology, Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, China.
推荐引用方式(GB/T 7714)：
Chen Yufang,Du Xing,Zhou Yande,et al.Paeoniflorin protects HUVECs from AGE-BSA-induced injury via an autophagic pathway by acting on the RAGE.[J].International journal of clinical and experimental pathology.2015,8(1):53-62.
APA：
Chen Yufang,Du Xing,Zhou Yande,Zhang Yanlin,Yang Yaping...&Xie Ying.(2015).Paeoniflorin protects HUVECs from AGE-BSA-induced injury via an autophagic pathway by acting on the RAGE..International journal of clinical and experimental pathology,8,(1)
MLA：
Chen Yufang,et al."Paeoniflorin protects HUVECs from AGE-BSA-induced injury via an autophagic pathway by acting on the RAGE.".International journal of clinical and experimental pathology 8..1(2015):53-62
