机构：[1]Department of Orthopedics, Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu 215004[2]Departments of Orthopedics , The First People's Hospital of Yancheng City, Yancheng, Jiangsu 224005, P.R. China[3]Departments of Laboratory Medicine, The First People's Hospital of Yancheng City, Yancheng, Jiangsu 224005, P.R. China
The aim of the present study was to investigate the association between the expression of nuclear receptor co-activator 5 protein (NCOA5) and the prognosis of postoperative patients with osteosarcoma. Human osteosarcoma samples were collected from 145 patients and normal bone tissues were collected from 100 individuals as controls. Immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) were employed to measure the levels of NCOA5 protein in cases of human osteosarcoma. The results from the RT-PCR analysis demonstrated that the positive rate of NCOA5 mRNA expression in human osteosarcoma was 17.24% (25/145). The positive rate in normal bone tissues was 84.00% (84/100), which was significantly higher compared with that of human osteosarcoma tissues (chi(2)=33.166; P<0.001). IHC staining indicated that the positive rate of NCOA5 protein in the osteosarcoma samples was 26.21% (38/145). The positive rate in normal bone tissues was 82.00% (82/100), which was significantly increased compared with that of human osteosarcoma tissues (chi(2)=28.166; P<0.001). NCOA5 mRNA and protein expression levels were consistent in human osteosarcoma tissues, and were lower than in control tissues. The expression of NCOA5 was low in human osteosarcoma tissues, while it was high in normal bone tissues. These low NCOA5 expression levels were associated with postoperative survival of human osteosarcoma.
基金：
The present study was supported by Jiangsu Pharmaceutical Association (grant no. 201542) and the Science and Technology commission of Yancheng City (grant no. YK2015002).
第一作者机构：[1]Department of Orthopedics, Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu 215004[2]Departments of Orthopedics , The First People's Hospital of Yancheng City, Yancheng, Jiangsu 224005, P.R. China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Orthopedics, Second Affiliated Hospital of Suzhou University, 1055 Sanxiang Road, Suzhou, Jiangsu 215004, P.R. China[*2]Department of Laboratory Medicine, The First People's Hospital of Yancheng City, 166 Yulong Road, Yancheng, Jiangsu 224005, P.R. China
推荐引用方式(GB/T 7714)：
YA WU,JIAN WU,QI-RONG DONG,et al.Association between expression of nuclear receptor co-activator 5 protein and prognosis in postoperative patients with osteosarcoma[J].ONCOLOGY LETTERS.2018,15(2):1888-1892.doi:10.3892/ol.2017.7513.
APA：
YA WU,JIAN WU,QI-RONG DONG&NAI-ZHOU GUO.(2018).Association between expression of nuclear receptor co-activator 5 protein and prognosis in postoperative patients with osteosarcoma.ONCOLOGY LETTERS,15,(2)
MLA：
YA WU,et al."Association between expression of nuclear receptor co-activator 5 protein and prognosis in postoperative patients with osteosarcoma".ONCOLOGY LETTERS 15..2(2018):1888-1892
