机构：[a]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou,[b]Osteoporosis Institute of Soochow University, Suzhou,[c]Department of Obstetrics and Gynaecology, The Second Affiliated Hospital of Soochow University, Suzhou, China
Background/Aims: Bone marrow mesenchymal stem cells (BMSCs) play an essential role in osteoporosis. However, the molecular mechanisms and the involvement of glutamine metabolism in osteogenic BMSCs differentiation and osteoporosis remain largely unclear. In this study, we investigated the role of Golgi membrane protein 1 (GOLM1) and glutamine metabolism in BMSCs differentiation and osteoporosis. Methods: Osteogenic differentiation-inducing media (Odi) was used to induce the osteogenic differentiation of BMSCs. The mRNA expression of GOLM1, ALP, Runx2, Osx, BSP and OCN was determined by qRT-PCR assay. Western blot assay was used to analyze GOLM1, p-mTOR, mTOR, p-S6 and S6 abundance in GOLM1 silencing and over-expressed BMSCs. Glutamine uptake, intracellular glutamine, glutamate and -KG level was detected using indicated Kits. GOLM1 antibody, glutamine metabolism inhibitors EGCG and BPTES were used to treat ovariectomy (OVX)-induced osteoporosis. Bone mineral density and bone volume relative to tissue volume (%) were analyzed by micro-CT. Serum was collected from osteoporosis patients and healthy participants and subjected to GOLM1 determination using ELISA Kit. Results: GOLM1 expression and glutamine metabolism were suppressed by Odi. GOLM1 blockage or inhibition of glutamine metabolism promoted the osteogenic differentiation of BMSCs induced by Odi. GOLM1 activated glutamine metabolism depending on the mTOR signaling pathway. In vivo, GOLM1 antibody or combination of glutamine inhibitor EGCG and BPTES rescued the osteoporosis in an OVX-operated mouse model. Serum GOLM1 level was increased in the patients of osteoporosis compared with healthy people. Conclusion: GOLM1 stimulates glutamine metabolism to suppress the osteogenic differentiation of BMSCs and to promote osteoporosis. Therefore, GOLM1 activation of glutamine metabolism is a potential target for osteoporosis.
基金：
This work was supported by Jiangsu Province’s Young Medical Talents Program
(QNRC2016880), National Natural Science Foundation of China Grant (81302438, 81572179)
and Advantage discipline groups of the Second Affiliated Hospital of Soochow University
(XKQ2015001).
第一作者机构：[a]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou,[b]Osteoporosis Institute of Soochow University, Suzhou,
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, 215004, Suzhou (China)
推荐引用方式(GB/T 7714)：
Guangsi Shen,Hong Zhang,Peng Jia,et al.GOLM1 Stimulation of Glutamine Metabolism Promotes Osteoporosis via Inhibiting Osteogenic Differentiation of BMSCs[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2018,50(5):1916-1928.doi:10.1159/000494872.
APA：
Guangsi Shen,Hong Zhang,Peng Jia,Guangfei Li,Xiao Wang...&Youjia Xu.(2018).GOLM1 Stimulation of Glutamine Metabolism Promotes Osteoporosis via Inhibiting Osteogenic Differentiation of BMSCs.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,50,(5)
MLA：
Guangsi Shen,et al."GOLM1 Stimulation of Glutamine Metabolism Promotes Osteoporosis via Inhibiting Osteogenic Differentiation of BMSCs".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 50..5(2018):1916-1928
