机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China[2]Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, New York 10065, USA[3]Public Health Research Institute TB Center, Rutgers University, Newark, New Jersey 07103, USA[4]Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou 215008, Jiangsu Province, China
The plasmid-encoded colistin resistance gene (mcr-1) has recently been reported in various Gram-negative species. However, the expression profile of mcr-1 remains unknown. Here, we investigated the expression of mcr-1 in various plasmids and bacteria. The mcr-1 expression levels in pMCR1_IncX4 varied from 1.81 x 10(-5) to 1.05 x 10(-4) (pmol per mu g total RNA) in the two K. pneumoniae strains SZ03 and SZ04 (ST25) and the two E. coli strains SZ01 and CDA6 (ST2448 and ST167, respectively). The mcr-1 expression levels of pMCR1_IncI2 in E. coli SZ02 (ST2085) and E. coli BJ13 (ST457) were 5.27 x 10(-5) and 2.58 x 10(-5), respectively. In addition, the expression of chromosomal mcr-1 in ST156 E. coli BJ10 was 5.49x10(-5). Interestingly, after 4 mu g/ml colistin treatment, mcr-1 in pMCR1_IncX4 increased 2- and 4-fold at 20 and 120 mins, respectively, in all pMCR1_IncX4-harboring strains, except for ST2448 E. coli, which had a lower expression after 20 mins that restored to baseline levels after 120 mins. In contrast, mcr-1 expression of pMCR1_IncI2 in the two E. coli strains (SZ02, BJ13) and the chromosomal mcr-1 in E. coli (BJ10) remained at baseline levels after 20 and 120 mins. In the same genetic background host strain E. coli E600, mcr-1 expression of pMCR1_IncX4 and pMCR1_IncI2 were similar and were decreased after colistin treatment for 20 min. However, mcr-1 in pMCR1_IncX4 was up-regulated after colistin treatment for 120 min, while mcr-1 in pMCR1_IncI2 was down-regulated compared to the untreated control. Our results suggested that mcr-1 has distinct expression profiles on different plasmids, bacterial hosts, and after antibiotic treatment.
基金：
This study was supported by the National Natural Science Foundation of China (81572032, 81401636), Key research and development project of Jiangsu provincial science and Technology Department (BE2017654), Six talent peaks project in Jiangsu Province (2016-WSN-112), Gusu key health talent of Suzhou, the Natural Science Foundation for Colleges and Universities in Jiangsu Province (16KJB320006), Jiangsu youth medical talents program (QN-866, 867) and the Science and Technology Program of Suzhou (SZS201715, SYS201551, SS201638, SYS201619).
第一作者机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China[3]Public Health Research Institute TB Center, Rutgers University, Newark, New Jersey 07103, USA[4]Department of Clinical Laboratory, The North District of Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou 215008, Jiangsu Province, China
推荐引用方式(GB/T 7714)：
Haifang Zhang,Minhui Miao,Jieting Yan,et al.Expression characteristics of the plasmid-borne mcr-1 colistin resistance gene[J].ONCOTARGET.2017,8(64):107596-107602.doi:10.18632/oncotarget.22538.
APA：
Haifang Zhang,Minhui Miao,Jieting Yan,Min Wang,Yi-Wei Tang...&Hong Du.(2017).Expression characteristics of the plasmid-borne mcr-1 colistin resistance gene.ONCOTARGET,8,(64)
MLA：
Haifang Zhang,et al."Expression characteristics of the plasmid-borne mcr-1 colistin resistance gene".ONCOTARGET 8..64(2017):107596-107602
医学