机构：[1]Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000[2]Department of Urology, Xishan People's Hospital of Wuxi, Wuxi, Jiangsu 214000[3]Department of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000[4]Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710061, P.R. China
Store-operated calcium entry (SOCE) plays an important role in the invasion and migration of cancer cells. Stromal-interacting molecule 1 (STIM1) is a critical component in the SOCE. STIM1 has been attracting more and more attention due to its oncogenic potential. STIM1 inhibition suppresses cell proliferation, migration and invasion in a variety of cancer models both in vitro and in vivo. However, the role of STIM1 in prostate carcinogenesis, in particular, in tumor migration and invasion is unclear. Herein, we downregulated STIM1 in prostate cancer cells by lentivirus-mediated short hairpin (shRNA), and then studied its impacts on cell migration and invasion. We found that migration and invasion of prostate cancer cells were significantly inhibited after the suppression of STIM1. Furthermore, we demonstrated that the PI3K/Akt signaling pathway was inactivated by STIM1 knockdown. The PI3K inhibitor LY294002 synergized with STIM1 knockdown to inhibit cell motility. Our results revealed that STIM1 may act as a novel regulator to promote migration and invasion of prostate cancer cells and is associated with the activation of the PI3K/Akt signaling pathway.
基金：
The present study was supported by the Foundation of Health
Bureau of Suzhou (SYSD2012084), the Natural Science
Foundation of Jiangsu Province (BK20161222) and the Suzhou
Foundation for the Development of Science and Technology
(SYS201629), P.R. China.
第一作者机构：[1]Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000
通讯作者：
通讯机构：[*1]Department of Urology, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu 215000, P.R. China
推荐引用方式(GB/T 7714)：
Yibin Zhou,Peng Gu,Jian Li,et al.Suppression of STIM1 inhibits the migration and invasion of human prostate cancer cells and is associated with PI3K/Akt signaling inactivation[J].ONCOLOGY REPORTS.2017,38(5):2629-2636.doi:10.3892/or.2017.5961.
APA：
Yibin Zhou,Peng Gu,Jian Li,Feng Li,Jin Zhu...&Dongrong Yang.(2017).Suppression of STIM1 inhibits the migration and invasion of human prostate cancer cells and is associated with PI3K/Akt signaling inactivation.ONCOLOGY REPORTS,38,(5)
MLA：
Yibin Zhou,et al."Suppression of STIM1 inhibits the migration and invasion of human prostate cancer cells and is associated with PI3K/Akt signaling inactivation".ONCOLOGY REPORTS 38..5(2017):2629-2636
