机构：[1]Affiliated Bayi Brain Hospital, P.L.A. Army General Hospital, Beijing 100700, China[2]Department of Neurosurgery, The 251st Hospital of P.L.A., Zhangjiakou 075000, China[3]Third Military Medical University, Chongqing 400038, China[4]Southern Medical University, Guangzhou 510515, China[5]School of Nursing, Binzhou Medical University, Yantai 264000, China[6]Institute of Neuroscience and Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Soochow University, Suzhou 215021, China[7]Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
Both the extrinsic environmental factors and intrinsic neuronal mechanisms limit the axonal regeneration after spinal cord injury (SCI). However, the underlying molecular mechanisms remain unclear. In the present study, we identify tripartite motif protein 32 (TRIM32), an E3 ubiquitin ligase, which is barely detected in glial cells in the normal uninjured spinal cord, exhibits strong expression in both astrocytes and microglia following SCI. We further observe that deficiency of TRIM32 results in increased numbers of astrocytes and microglia, which is accompanied by enhanced proliferation of both cells and increased secretion of interleukin (IL)-1 and IL-10. The axonal regeneration is impaired in the spinal cord of TRIM32(-/-)mice following SCI, which is indicated by increased distances of the corticospinal tracts (CST) fiber to the lesion site and less axonal sprouting. We further show that deficiency of TRIM32 results in delay motor recovery following SCI. Therefore, TRIM32 is a novel essential positive factor modulating axonal regeneration and the recovery of motor function following SCI, possibly through suppressing proliferation of glial cells.
基金：
This work was supported by the National Natural Science Foundation of China (81671111, 81501950), the Military Medical Science Research Project (16CXZ001), the National Program on Key Basic Research Project (2013CB945602), the Suzhou Clinical Research Center of Neurological Disease (Szzx201503) and Jiangsu Provincial Special Program of Medical Science (BL2014042) and to the Jiangsu Higher Education Institutions for funding the Priority Academic Program Development; Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases (BM2013003).
第一作者机构：[1]Affiliated Bayi Brain Hospital, P.L.A. Army General Hospital, Beijing 100700, China[2]Department of Neurosurgery, The 251st Hospital of P.L.A., Zhangjiakou 075000, China
共同第一作者：
通讯作者：
通讯机构：[1]Affiliated Bayi Brain Hospital, P.L.A. Army General Hospital, Beijing 100700, China[3]Third Military Medical University, Chongqing 400038, China[4]Southern Medical University, Guangzhou 510515, China[6]Institute of Neuroscience and Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Soochow University, Suzhou 215021, China[7]Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
推荐引用方式(GB/T 7714)：
Qiang Fu,Ming-Ming Zou,Jian-Wei Zhu,et al.TRIM32 affects the recovery of motor function following spinal cord injury through regulating proliferation of glia[J].ONCOTARGET.2017,8(28):45380-45390.doi:10.18632/oncotarget.17492.
APA：
Qiang Fu,Ming-Ming Zou,Jian-Wei Zhu,Yan Zhang,Wen-Jin Chen...&Ru-Xiang Xu.(2017).TRIM32 affects the recovery of motor function following spinal cord injury through regulating proliferation of glia.ONCOTARGET,8,(28)
MLA：
Qiang Fu,et al."TRIM32 affects the recovery of motor function following spinal cord injury through regulating proliferation of glia".ONCOTARGET 8..28(2017):45380-45390
医学