机构：[1]School of Biology and Basic Medical Sciences and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China[2]National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, People’s Republic of China[3]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215000, People’s Republic of China[4]Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, United States
Injectable hydrogel systems are important bone substitutes for regeneration because of their handling properties and the ability to fill irregular defects. Silk-hydroxyapatite composite materials with silk nanofibers in hydrogels were prepared and used as biomaterials for osteogenesis. These thixotropic silk nanofiber hydrogels and water-dispersible silk-HA nanoparticles were blended to form injectable nanoscale systems with a homogeneous distribution of a high HA content [60% (w/w)] to imitate bone niche. A modulus of similar to 21 kPa was also achieved following the addition of HA in the systems, providing physical cues to induce osteodifferentiation. The composite hydrogels supported improved osteogenesis compared to that with silk nanofiber hydrogels. The newly formed bone tissue and bone defect healing were detected after implantation of the silk-HA composite hydrogels, suggesting utility for the regeneration of irregular bone defects.
基金：
The authors thank the National Basic Research Program of
China (973 Program, 2013CB934400), NSFC (81272106), and
the National Institutes of Health (R01 DE017207 and P41
EB002520). The authors also thank the Natural Science
Foundation of Jiangsu Province (Grants BK20140397 and
BK20140401) and the second affiliated hospital of Soochow
University preponderant clinic discipline group project funding
(NO.XKQ2015010) for support of this work.
第一作者机构：[1]School of Biology and Basic Medical Sciences and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China[2]National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]School of Biology and Basic Medical Sciences and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People’s Republic of China[2]National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, People’s Republic of China
推荐引用方式(GB/T 7714)：
Zhaozhao Ding ,Hongyan Han ,Zhihai Fan ,et al.Nanoscale Silk-Hydroxyapatite Hydrogels for Injectable Bone Biomaterials[J].ACS APPLIED MATERIALS & INTERFACES.2017,9(20):16913-16921.doi:10.1021/acsami.7b03932.
APA：
Zhaozhao Ding,,Hongyan Han,,Zhihai Fan,,Haijun Lu,,Yonghuan Sang,...&David L. Kaplan.(2017).Nanoscale Silk-Hydroxyapatite Hydrogels for Injectable Bone Biomaterials.ACS APPLIED MATERIALS & INTERFACES,9,(20)
MLA：
Zhaozhao Ding,,et al."Nanoscale Silk-Hydroxyapatite Hydrogels for Injectable Bone Biomaterials".ACS APPLIED MATERIALS & INTERFACES 9..20(2017):16913-16921
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