机构：[1]Center of Stomatology, The Second Affiliated Hospital of Soochow University , Suzhou 215004, People's Republic of China. [2]State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, People's Republic of China. [3]Xi'an Jiaotong University Suzhou Research Institute , Suzhou 215123, People's Republic of China. [4]Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University , Nanjing 210029, People's Republic of China.
To improve bone regeneration in oral microenvironment, we generated a novel biodegradable, antibacterial, and osteoconductive electrospun PLGA/PCL membrane as an ideal osteogenic scaffold. The novel three-layer membranes were structured with serial layers of electrospun chlorhexidine-doped-PLGA/PCL (PPC), PLGA/PCL (PP), and beta-tricalcium phosphate-doped-PLGA/PCL (PP beta). To characterize osteoconductive properties of these membranes, MC3T3-E1 (MC) cultures were seeded onto the membranes for 14 days for evaluation of cell proliferation, morphology and gene/protein expression. In addition, MC cells were cultured onto different surfaces of the three-layer membranes, PPC layer facing MC cells (PP beta-PP-PPC) and PP beta layer facing MC cells (PPC-PP-PP beta) to evaluate surfacematerial effects. Membrane properties and structures were evaluated. Antibacterial properties against Streptococcus mutans and Staphylococcus aureus were determined. Scanning electron microscope demonstrated smaller interfiber spaces of PPC and PP beta-PP-PPC compared to PP beta, PPC-PP-PP beta, and PP. PPC and PP beta-PP-PPC exhibited hydrophilic property. The three-layer membranes (PPC-PP-PP beta and PP beta-PP-PPC) demonstrated significantly higher Youngs modulus (94.99 +/- 4.03 MPa and 92.88 +/- 4.03 MPa) compared to PP (48.76 +/- 18.15 MPa) or PPC (7.92 +/- 3.97 MPa) (p < 0.05). No significant difference of cell proliferation was found among any groups at any time point (p > 0.05). Higher expression of integrins were detected at 12 h of cultures on PPC-PP-PP beta compared to the controls. Promoted osteoconductive effects of PPC-PP-PP beta were revealed by alkaline phosphatase assays and Western blot compared with the controls at 7 and 14 days. PPC, PPC-PP-PP beta and PP beta-PP-PPC exhibited a significantly wider antibacterial zone against the tested bacteria compared to PP and PP beta (p < 0.05). These results suggested that the three-layer electrospun membranes demonstrated superior properties: higher strength, better cell adhesion, and promoted osteoconductive properties compared to single-layer membrane: however, antibacterial properties were exhibited in three-layer electrospun membranes and chlorhexidine-doped single-layer membrane. We concluded that the novel three-layer membranes could be used as a biocompatible scaffold for intraoral bone regeneration due to its enhanced osteoconductive activity and antibacterial effect.
基金：
This work was supported by the National Key Research and
Development Plan (Grant Nos. 2016YFA0201704 and
2017YFA0104301), the National Natural Science Foundation
of China (Grant No. 31200757), Suzhou Health and Family
Planning Commission (Grant No. SYSD2015093), the Preponderant Clinic Discipline Group Project Funding of the
Second Affiliated Hospital of Soochow University (Grant No.
XKQ2015010), Advance Research Program of the Second
Affiliated Hospital of Soochow University (Grant No.
SDFEYBS1603), and Collaborative Innovation Center of
Suzhou Nano Science and Technology. We are all grateful to
Dr. Phimon Atsawasuwan (Associate Professor, College of
Dentistry, University of Illinois at Chicago) for his valued help
with English editing
第一作者机构：[1]Center of Stomatology, The Second Affiliated Hospital of Soochow University , Suzhou 215004, People's Republic of China.
通讯作者：
推荐引用方式(GB/T 7714)：
Yunzhu Qian ,Xuefeng Zhou ,Hong Sun ,et al.Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone Regeneration Synergistically with Antibacterial Efficacy for Bone Defects[J].ACS APPLIED MATERIALS & INTERFACES.2018,10(4):3248-3259.doi:10.1021/acsami.7b14524.
APA：
Yunzhu Qian,,Xuefeng Zhou,,Hong Sun,,Jianxin Yang,,Yi Chen,...&Ning Gu.(2018).Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone Regeneration Synergistically with Antibacterial Efficacy for Bone Defects.ACS APPLIED MATERIALS & INTERFACES,10,(4)
MLA：
Yunzhu Qian,,et al."Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone Regeneration Synergistically with Antibacterial Efficacy for Bone Defects".ACS APPLIED MATERIALS & INTERFACES 10..4(2018):3248-3259
工程技术