机构：[a]School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China[b]Xuanwu Hospital, Capital Medical University, Beijing 100053, P. R. China首都医科大学宣武医院
A biodegradable poly(epsilon-caprolactone) (PCL) was synthesized by ring-opening polymerization of epsilon-caprolactone catalyzed by Sn(Oct)(2)/BDO, followed by the heparin conjugation using EDC/NHS chemistry. The structure of the heparin-PCL conjugate was characterized by H-1-NMR and GPC. The results of static contact angle and water uptake ratio measurements also confirmed the conjugation of heparin with the polyester. Its in vitro anticoagulation time was substantially extended, as evidenced by activated partial thromboplastin time (APTT) testing. Afterwards the conjugate was electrospun into small-diameter tubular scaffolds and loaded with Fibroblast Growth Factor 2 (FGF2) in aqueous solution. The loading efficiency was assayed by enzyme-linked immunosorbent assay (ELISA); the results indicated that the conjugate holds a higher loading efficiency than the blank polyester. The viability of released FGF2 was evaluated by MTT and cell adhesion tests. The amount and morphology of cells were significantly improved after FGF2 loading onto the electrospun heparin-PCL vascular scaffolds. (c) Koninklijke Brill NV, Leiden, 2011
基金：
the ‘863’ Project of the Ministry of Science and Technology of China (No. 2006AA02A134 and 2007AA021905) and the Basic Research Foundation of the Beijing Institute of Technology (20080942001).
第一作者机构：[a]School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China
通讯作者：
通讯机构：[a]School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China
推荐引用方式(GB/T 7714)：
Lin Ye,XinWu,Qian Mu,et al.Heparin-Conjugated PCL Scaffolds Fabricated by Electrospinning and Loaded with Fibroblast Growth Factor 2[J].JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION.2011,22(1-3):389-406.doi:10.1163/092050610X487710.
APA：
Lin Ye,XinWu,Qian Mu,Bing Chen,Yonghong Duan...&Zeng-guo Feng.(2011).Heparin-Conjugated PCL Scaffolds Fabricated by Electrospinning and Loaded with Fibroblast Growth Factor 2.JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,22,(1-3)
MLA：
Lin Ye,et al."Heparin-Conjugated PCL Scaffolds Fabricated by Electrospinning and Loaded with Fibroblast Growth Factor 2".JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 22..1-3(2011):389-406
