机构：[1]Departments of Molecular Biology Laboratory,Shanghai Centre for Clinical Laboratory, Shanghai 200126[2]Departments of Microbiology Laboratory, Shanghai Centre for Clinical Laboratory, Shanghai 200126[3]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[4]Department of Clinical Laboratory, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200233, P.R. China
This study aimed to investigate antibiotic resistance genes in the multidrug-resistant (MDR) Acinetobacter baumannii (A. baumanii) strain, MDR-SHH02, using whole-genome sequencing (WGS). The antibiotic resistance of MDR-SHH02 isolated from a patient with breast cancer to 19 types of antibiotics was determined using the Kirby-Bauer method. WGS of MDR-SHH02 was then performed. Following quality control and transcriptome assembly, functional annotation of genes was conducted, and the phylogenetic tree of MDR-SHH02, along with another 5 A. baumanii species and 2 Acinetobacter species, was constructed using PHYLIP 3.695 and FigTree v1.4.2. Furthermore, pathogenicity islands (PAIs) were predicted by the pathogenicity island database. Potential antibiotic resistance genes in MDR-SHH02 were predicted based on the information in the Antibiotic Resistance Genes Database (ARDB). MDR-SHH02 was found to be resistant to all of the tested antibiotics..The total draft genome length of MDR-SHHO2 was 4,003,808 bp. There were 74.25% of coding sequences to be annotated into 21 of the Clusters of Orthologous Groups (COGs) of protein terms, such as 'transcription' and `amino acid transport and metabolism'. Furthermore, there were 45 PAIs homologous to the sequence MDRSHH02000806. Additionally, a total of 12 gene sequences in MDR-SHH02 were highly similar to the sequences of antibiotic resistance genes in ARDB, including genes encoding aminoglycoside-modifying enzymes [e.g., aac(3)-Ia, ant(2 '')-Ia, aph33ib and aph(3')-Ia], beta-lactamase genes (b12b_tem and bl2b_teml), sulfonamide resistant dihydropteroate synthase genes (sull and sul2), catb3 and tetb. These results suggest that numerous genes mediate resistance to various antibiotics in MDR-SHH02, and provide a clinical guidance for the personalized therapy of A. baumannii-infected patients.
基金：
This study was supported by a project supported by Shanghai Health and Family Planning Commission Foundation, China (grant no. 20134010) and a project supported by the Natural Science Foundation of Shanghai, China (grant no. 15ZR1436100).
第一作者机构：[1]Departments of Molecular Biology Laboratory,Shanghai Centre for Clinical Laboratory, Shanghai 200126[*1]Department of Molecular Biology Laboratory, Shanghai Center for Clinical Laboratory, 528 Hongshan Road, Pudong New Area, Shanghai 200126, P.R. China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Molecular Biology Laboratory, Shanghai Center for Clinical Laboratory, 528 Hongshan Road, Pudong New Area, Shanghai 200126, P.R. China
推荐引用方式(GB/T 7714)：
HUALIANG WANG,JINGHUA WANG,PEIJUAN YU,et al.Identification of antibiotic resistance genes in the multidrug-resistant Acinetobacter baumannii strain, MDR-SHH02, using whole-genome sequencing[J].INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE.2017,39(2):364-372.doi:10.3892/ijmm.2016.2844.
APA：
HUALIANG WANG,JINGHUA WANG,PEIJUAN YU,PING GE,YANQUN JIANG...&XUEJIE LIU.(2017).Identification of antibiotic resistance genes in the multidrug-resistant Acinetobacter baumannii strain, MDR-SHH02, using whole-genome sequencing.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,39,(2)
MLA：
HUALIANG WANG,et al."Identification of antibiotic resistance genes in the multidrug-resistant Acinetobacter baumannii strain, MDR-SHH02, using whole-genome sequencing".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 39..2(2017):364-372
