机构：[a]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China[b]Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China
The objective of this work was to develop a simple, selective, and sensitive LC-MS/MS method for the quantitation of the mepivacaine in Chinese biological matrix. The calibration curve of mepivacaine ranged from 0.5 to 2000ng/mL with the lower limit of quantitation being 0.5ng/mL. This sensitivity was high enough to describe the profile of blood mepivacaine level versus time. Thereby it was very desirable for the pharmacokinetic study because of its high sensitivity and accuracy. The study used a single-dose two-period crossover design principle. For the pharmacokinetic analysis of plasma, the mean (SD) values obtained were as follows: t(1/2), 1.63 (0.43) h; C-max, 435.3 (67.4) ng/ml; AUC(0-t), 1546.9 (339.7) ng/ml<bold>h</bold>; AUC(0-), 1982.3 (421.4) ng/ml<bold>h</bold>; T-max, 0.62 (0.31) h. The validated method has been successfully applied to assess the pharmacokinetic study of mepivacaine after a single administration to Chinese volunteers.
第一作者机构：[a]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China[b]Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China
共同第一作者：
通讯作者：
通讯机构：[*1]1055 Sanxiang Road, 215004 Suzhou, China
推荐引用方式(GB/T 7714)：
Ruo-Wang Duan,Jiong Song,Yu-Ping Li,et al.A novel LC-MS/MS method for mepivacaine determination and pharmacokinetic study in a single-dose two-period crossover in healthy subjects[J].ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY.2017,45(8):1605-1611.doi:10.1080/21691401.2016.1267013.
APA：
Ruo-Wang Duan,Jiong Song,Yu-Ping Li&Chun-Gen Xing.(2017).A novel LC-MS/MS method for mepivacaine determination and pharmacokinetic study in a single-dose two-period crossover in healthy subjects.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,45,(8)
MLA：
Ruo-Wang Duan,et al."A novel LC-MS/MS method for mepivacaine determination and pharmacokinetic study in a single-dose two-period crossover in healthy subjects".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 45..8(2017):1605-1611
