机构：[a]Department of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[b]Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China[c]Institute of Neuroscience, Soochow University, Suzhou 215123, China
Foam cell formation, which is caused by imbalanced cholesterol influx and efflux by macrophages, plays a vital role in the occurrence and development of atherosclerosis. Humanin (HN), a mitochondria-derived peptide, can prevent the production of reactive oxygen species and death of human aortic endothelial cells exposed to oxidized low-density lipoprotein (ox-LDL) and has a protective effect on patients with in early atherosclerosis. However, the effects of HN on the regulation of cholesterol metabolism in RAW 264.7 macrophages are still unknown. This study was designed to investigate the role of [G1y14]humanin (HNG) in lipid uptake and cholesterol efflux in RAW 264.7 macrophages. Flow cytometry and live cell imaging results showed that HNG reduced Dil-ox-LDL accumulation in the RAW 264.7 macrophages. A similar result was obtained for lipid accumulation by measuring cellular cholesterol content. Western blot analysis showed that ox-LDL treatment upregulated not only the protein expression of CD36 and LOX-1, which mediate ox-LDL endocytosis, but also ATP-binding cassette (ABC) transporter A1 and ABCG1, which mediate ox-LDL exflux. HNG pretreatment inhibited the upregulation of CD36 and LOX-1 levels, prompting the upregulation of ABCA1 and ABCG1 levels induced by ox-LDL. Therefore we concluded that HNG could inhibit ox-LDL-induced macrophage-derived foam cell formation, which occurs because of a decrease in lipid uptake and an increase in cholesterol efflux from macrophage cells. (C) 2016 Elsevier Inc. All rights reserved.
基金：
This work was supported by a grant from the Natural
Science Foundation of Jiangsu Province (no. BK20122172), the National
Natural Science Foundation of China (nos. 81200894,
81471195, and 81670742), Suzhou Foundation of Science and
Technology Development Plan (nos. SYSD201548) and a project
funded by the Priority Academic Program Development of Jiangsu
Higher Education Institutions (PAPD); and this project is also subject
to the second affiliated hospital of Soochow university preponderant
clinic discipline group project funding (no.XKQ2015002).
第一作者机构：[a]Department of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[a]Department of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[*1]Department of Neurology, Second Affiliated Hospital of Suzhou University, 1055 Sanxiang Road, Suzhou 215004, China.
推荐引用方式(GB/T 7714)：
Wa-wa Zhu,Shu-rongWang,Zhi-hua Liu,et al.Gly[14]-humanin inhibits ox-LDL uptake and stimulates cholesterol efflux in macrophage-derived foam cells[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2017,482(1):93-99.doi:10.1016/j.bbrc.2016.10.138.
APA：
Wa-wa Zhu,Shu-rongWang,Zhi-hua Liu,Yong-jun Cao,FenWang...&Yan-lin Zhang.(2017).Gly[14]-humanin inhibits ox-LDL uptake and stimulates cholesterol efflux in macrophage-derived foam cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,482,(1)
MLA：
Wa-wa Zhu,et al."Gly[14]-humanin inhibits ox-LDL uptake and stimulates cholesterol efflux in macrophage-derived foam cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 482..1(2017):93-99
