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【已隐藏】Interleukin-1 beta Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1

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收录情况： ◇ SCIE

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机构： [1]Department of Nephrology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China [2]Department of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

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关键词： Interleukin-1 beta oxidised low-density lipoprotein lectin-like Ox-LDL receptor 1

摘要：

The aim of this study was to determine whether interleukin-1 beta (IL-1 beta) promotes oxidised low-density lipoprotein (Ox-LDL) uptake by human glomerular mesangial cells (HMCs) and its effect on the expression of lectin-like Ox-LDL receptor 1 (LOX-1) and to identify pathways through which IL - 1 beta affects lipid uptake. Confocal laser scanning microscopy and flow cytometry were used to observe the effect of IL-1 beta on lipid uptake by HMCs and the pathway by which IL-1 beta may mediate lipid uptake. Real-time polymerase chain reaction (PCR) and western blotting were used to evaluate the effect of IL - 1 beta on LOX-1 expression. Confocal laser scanning microscopy and flow cytometry revealed that IL-1 beta promoted uptake of fluorescent Dil-labelled Ox-LDL(Dil-Ox-LDL) by HMCs and the enhanced uptake of Dil-Ox-LDL was partially inhibited by an anti-LOX-1 antibody evaluated by flow cytometry. Further, IL - 1 beta promoted LOX-1 mRNA and protein expression of HMCs in a dose- and time-dependent manner. Thus, Ox-LDL is ingested by HMCs under basic conditions. Inflammatory cytokine IL-1 beta promotes Ox-LDL uptake by HMCs. Furthermore, IL-1 beta promotes the mRNA and protein expression of LOX-1, a specific receptor of Ox-LDL, suggesting that the enhancement of Ox-LDL uptake may be mediated by LOX-1 pathway. Anti-LOX-1 therapy may be a promising option for treatment of glomerulosclerosis.

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出版当年[2019]版：

大类 | 3 区医学

小类 | 3 区医学：内科

最新[2023]版：

大类 | 3 区医学

小类 | 3 区医学：内科

JCR分区：

出版当年[2018]版：

Q2 MEDICINE, GENERAL & INTERNAL

最新[2023]版：

Q1 MEDICINE, GENERAL & INTERNAL

影响因子： 3.2 最新[2023版] 3.4 最新五年平均 2.333 出版当年[2018版] 2.636 出版当年五年平均 2.284 出版前一年[2017版] 2.523 出版后一年[2019版]

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第一作者机构： [1]Department of Nephrology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

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