机构：[a]Department of Ophthalmology, Daqing Oil General Hospital, Daqing, People’s Republic of China[b]Department of Ophthalmology, Daqing People’s Hospital, Daqing, People’s Republic of China[c]Department of Ophthalmology, Secondary People’s Hospital of Mudanjiang City, Mudanjiang City, People’s Republic of China[d]Department of Opthalmology, Dashiqiao Central Hospital, Yingkou, People’s Republic of China[e]Department of Ophthalmology, Beijing Tiantan Hospital Affiliated to the Capital Medical University, Beijing, People’s Republic of China诊疗科室眼科首都医科大学附属天坛医院[f]Department of Ophthalmology, Shenyang Red Cross Hospital, Shenyang, People’s Republic of China
The abuse of 3,4-methylenedioxymethamphetamine (MDMA), a psychedelic drug, can lead to a variety of disorders in neural system, including the death of retinal neural cells. MDMA at lower doses does not cause obvious cytotoxicity to photoreceptor cells, indicating potential indirect mechanisms which have not yet been elucidated. This study investigated the effect of MDMA at nontoxic concentration on macrophage activation state and its resultant toxicity to photoreceptor cells. Using a co-culture system, cytotoxicity was caused by MDMA on 661W cells after co-culturing with RAW264.7 macrophage. Results showed that MDMA induced the macrophages to M1 polarization, releasing more pro-inflammatory cytokines, upregulating the M1-related gene and protein expression. The phenotype, secretion pattern, and cytotoxicity of the macrophages treated by MDMA are comparable to those of the ones stimulated by IFN and LPS. Our study demonstrated that MDMA promoted macrophage polarization to M1 and induced inflammatory response, providing the scientific rationale for the photoreceptor cell damage caused by the MDMA abuse.
第一作者机构：[a]Department of Ophthalmology, Daqing Oil General Hospital, Daqing, People’s Republic of China[*1]Department of Ophthalmology, Daqing Oil General Hospital, Daqing, People’s Republic of China
通讯作者：
通讯机构：[a]Department of Ophthalmology, Daqing Oil General Hospital, Daqing, People’s Republic of China[*1]Department of Ophthalmology, Daqing Oil General Hospital, Daqing, People’s Republic of China
推荐引用方式(GB/T 7714)：
Xin Liu,Li-Hui Zhan,Xiao-Hong Sun,et al.3,4-Methylenedioxymethamphetamine causes cytotoxicity on 661W cells through inducing macrophage polarization[J].CUTANEOUS AND OCULAR TOXICOLOGY.2018,37(2):143-150.doi:10.1080/15569527.2017.1359838.
APA：
Xin Liu,Li-Hui Zhan,Xiao-Hong Sun,Tao Zhang,Zhi-Li Liu...&Chun-Sheng Luan.(2018).3,4-Methylenedioxymethamphetamine causes cytotoxicity on 661W cells through inducing macrophage polarization.CUTANEOUS AND OCULAR TOXICOLOGY,37,(2)
MLA：
Xin Liu,et al."3,4-Methylenedioxymethamphetamine causes cytotoxicity on 661W cells through inducing macrophage polarization".CUTANEOUS AND OCULAR TOXICOLOGY 37..2(2018):143-150
