机构：[1]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004[2]Department of Neurosurgery, Mianyang Central Hospital, Mianyang, Sichuan 621000[3]School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123[4]Department of Neurosurgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450000, P.R. China
Maspin, a member of the serpin superfamily of serine protease inhibitors, has been reported to be involved in cancer initiation and progression. However, the expression of maspin and its expression regulation in glioma remain unknown. In the present study, we aimed to investigate the function of maspin in glioma cells and its regulatory mechanism. We found that the expression of maspin was silenced in glioma cells and tissues. Although maspin had no effect on the migration and invasion of human glioma cells in vitro, overexpression of maspin inhibited cell growth in U87 cells. We showed that the methylase inhibitor 5-Aza-2'-deoxycytidine induced the expression of maspin in glioma cell lines. Furthermore, both U87 and U251 cells showed hypermethylation in the maspin promoter. In addition, bisulphite sequencing analysis indicated that 16 CpG sites in the promoter were completely methylated in glioma cells and cancerous tissues, while CpG dinucleotides in the maspin promoter were unmethylated in normal brain tissues. Our data suggest that methylation-induced silencing of maspin contributes to the proliferation of human glioma cells, and maspin may be a potential therapeutic target in glioma.
基金：
This study was supported by grants from the National
Natural Science Foundation of China (81072058), the Natural
Science Foundation of Jiangsu Province (BK2010230), the
Foundation Program of Suzhou Science and Technology
Project (SYSD2014092), Research and Innovation Project
for College Graduates of Jiangsu Province (2014-1263), and the Foundation of Young Member of the Second Affiliated
Hospital of Soochow University (SDFEYQN1409).
第一作者机构：[1]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurosurgery, Henan Provincial People's Hospital, 7 Weiwu Road, Zhengzhou, Henan 450000, P.R. China[*2]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu 215004, P.R. China
推荐引用方式(GB/T 7714)：
LIANG XU,HONGYUAN LIU,JU YU,et al.Methylation-induced silencing of maspin contributes to the proliferation of human glioma cells[J].ONCOLOGY REPORTS.2016,36(1):57-64.doi:10.3892/or.2016.4783.
APA：
LIANG XU,HONGYUAN LIU,JU YU,ZHONGYONG WANG,QING ZHU...&QING LAN.(2016).Methylation-induced silencing of maspin contributes to the proliferation of human glioma cells.ONCOLOGY REPORTS,36,(1)
MLA：
LIANG XU,et al."Methylation-induced silencing of maspin contributes to the proliferation of human glioma cells".ONCOLOGY REPORTS 36..1(2016):57-64
