机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, China.[2]Department of Clinical Laboratory, Suqian Center of Disease Control and Prevention, Suqian, Jiangsu, China.[3]Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Jiangsu is one province with severe HIV-1 epidemic in China. However, the molecular epidemiological characterizations of HIV-1 in many cities of Jiangsu remain unclear. A molecular epidemiological investigation was performed based on 38 HIV-positive samples collected from Suzhou and Suqian during 2011-2013. Five HIV-1 genomic fragments, p17, pol, vif-vpr, vpr-env, and C2V3 were amplified and sequenced from these samples. HIV-1 group M subtype of each sample was determined by phylogenetic analyses with the standard reference sequences. Among these infected individuals, 81.6 % (31/38) self-reported to be infected via sexual contacts, including 50.0 % (19/38) via heterosexual contact and 31.6 % (12/38) via homosexual contact. Among 34 samples with available pol or vif-env sequence, 19 (55.9 %) CRF01_AE, 7 (20.6 %) CRF07_BC, 3 (8.8 %) CRF08_BC, and 5 (14.7 %) inter-subtype recombinants were identified. No pure B, B' and C subtypes were found in this cohort. The five recombinants contain one B/C, three CRF01/B and one CRF01/B/C recombinants. These results suggest that CRF01_AE was the most predominant HIV-1 group M subtype and CRF01_AE-involved recombinants were the major recombinant forms. Comparison showed that there was no obvious difference in HIV-1 group M subtype distribution between Jiangsu (including Suzhou and Suqian) and the surrounding provinces (e.g., Shanghai, Anhui, and Shandong). CRF01_AE and CRF07_BC were the top two predominant HIV-1 genotypes in Jiangsu, and less and/or no pure subtype B and C was currently circulating here. We predicted that more CRF01/CRF07 recombinants, but fewer B/C recombinants will be generated in Jiangsu in future.
基金：
This study was supported by the startup funds from the Second Affiliated
Hospital of Soochow University (No. SDFEYGJ1301).
第一作者机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou 215004, Jiangsu, China.
推荐引用方式(GB/T 7714)：
Chenhao Qin,Ping Zhang,Weiguang Zhu,et al.HIV-1 diversity in infected individuals in Suzhou and Suqian, China[J].SPRINGERPLUS.2016,5(1):886.doi:10.1186/s40064-016-2378-z.
APA：
Chenhao Qin,Ping Zhang,Weiguang Zhu,Fangyuan Hao,Aiping Gu...&Hong Du.(2016).HIV-1 diversity in infected individuals in Suzhou and Suqian, China.SPRINGERPLUS,5,(1)
MLA：
Chenhao Qin,et al."HIV-1 diversity in infected individuals in Suzhou and Suqian, China".SPRINGERPLUS 5..1(2016):886
