机构：[1]Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003[2]Department of Pediatric Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025[3]Department of Pediatric Surgery, Ruijin Hospital North, Shanghai Jiaotong University School of Medicine, Shanghai 201821[4]Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu 215004, P.R. China
The histone deacetylase (HDAC) family is comprised of enzymes, which are involved in modulating the majority of critical cellular processes, including transcriptional regulation, apoptosis, proliferation and cell cycle progression. However, the biological function of HDAC5 in Wilms' tumor remains to be fully elucidated. The present study aimed to investigate the expression and function of HDAC5 in Wilm's tumor. It was demonstrated that the mRNA and protein levels of HDAC5 were upregulated in human Wilms' tumor tissues. Overexpression of HDAC5 in G401 cells was observed to significantly promote cellular proliferation, as demonstrated by the results of an MTT assay and bromodeoxyuridine incorporation assay. By contrast, HDAC5 knockdown using small interfering RNA suppressed the proliferation of the G401 cells. At the molecular level, the present study demonstrated that HDAC5 promoted the expression of c-Met, which has been previously identified as an oncogene. In addition, downregulation of c-Met inhibited the proliferative effects of HDAC5 in human Wilms' tumor cells. Taken together, these results suggested that HDAC5 promotes cellular proliferation through the upregulation of c-Met, and may provide a novel therapeutic target for the treatment of patients with Wilms' tumor.
基金：
The present study was supported by grants from the Science Foundation of Jiangsu Province Health Department (grant nos. Q201304 and Q201503), the Natural Science Foundation of Jiangsu Province of China (grant nos. BK2011312 and BL2012051) and the Natural Science Foundation of China (no. 81502496).
第一作者机构：[1]Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, 330 Jingde Road, Suzhou, Jiangsu 215003, P.R. China
推荐引用方式(GB/T 7714)：
XU CAO,DE-HONG LIU,YUN ZHOU,et al.Histone deacetylase 5 promotes Wilms' tumor cell proliferation through the upregulation of c-Met[J].MOLECULAR MEDICINE REPORTS.2016,13(3):2745-50.doi:10.3892/mmr.2016.4828.
APA：
XU CAO,DE-HONG LIU,YUN ZHOU,XIANG-MING YAN,LI-QUN YUAN...&JIAN WANG.(2016).Histone deacetylase 5 promotes Wilms' tumor cell proliferation through the upregulation of c-Met.MOLECULAR MEDICINE REPORTS,13,(3)
MLA：
XU CAO,et al."Histone deacetylase 5 promotes Wilms' tumor cell proliferation through the upregulation of c-Met".MOLECULAR MEDICINE REPORTS 13..3(2016):2745-50
